It's not clear how fermentation affects the succession and interspecific interaction of functional microorganism in primary sludge anaerobic fermentation system. So as to explore the decisive role of temperature on microorganisms to reveal the mechanism, four fermentation experiments groups (25, 35, 45, and 55 °C) were designed. Results indicated the promotion in proportion of acetic and iso-valeric acid with temperature increasing. High-throughput sequencing reflected that the dominant microbial groups in all samples were Proteobacteria, Firmicutes, Bacteroidetes, Synergetes, Spirochaetae, and then key microbial interspecific interaction changed significantly due to the adjustment of microbial temperature. In addition, amino acid metabolism was the lowest at 35 °C, but carbohydrate metabolism was the highest. The metabolic abundances of amino acids at different temperatures were the highest, but the differences among kind of amino acids were significant. This study could reveal the effect of temperature on VFAs production in terms of microbial interspecific interaction and lay a theoretical foundation for sludge fermentation.

目前尚不清楚发酵对初级污泥厌氧发酵系统中功能微生物的演替和种间相互作用的影响。为探究温度对微生物的决定作用，揭示其作用机制，设计了四个发酵实验组（25、35、45和55℃）。结果表明，随着温度的升高，乙酸和异戊酸的比例增加。高通量测序反映所有样品中优势微生物群为变形菌门、厚壁菌门、拟杆菌门、协同门、螺菌门，然后关键微生物种间相互作用因微生物温度的调整而发生显着变化。此外，35℃时氨基酸代谢最低，碳水化合物代谢最高。不同温度下氨基酸的代谢丰度最高，但氨基酸种类间差异显着。该研究可以从微生物种间相互作用的角度揭示温度对VFAs产生的影响，为污泥发酵奠定理论基础。