Food and Agricultural Immunology ( IF 1.7 ) Pub Date : 2021-09-19 , DOI: 10.1080/09540105.2021.1972939 Na Gyeong Geum 1 , Ju-Hyeong Yu 1 , Joo Ho Yeo 1 , Min Yeong Choi 1 , Yurry Um 2 , Hong Woo Park 2 , Kiyoon Kim 2 , Jae Won Lee 1 , Jueng Kyu Beak 1 , Kwon Seok Jeon 2 , Jin Boo Jeong 1
ABSTRACT
We evaluated in vitro anti-obesity and immunostimulatory activity of wood cultivated ginseng. The roots (WCG-R) and stems (WCG-S) of wood cultivated ginseng reduced the lipid accumulation and TG contents in 3T3-L1 cells. WCG-R and WCG-S inhibited the protein expressions such as CEBPα, PPARγ, perilipin-1, adiponectin and FABP4 related to the lipid accumulation of the mature adipocytes. In addition, WCG-R and WCG-S reduced CEBPα and PPARγ during the differentiation process from preadipocytes to mature adipocytes. WCG-S increased the production of immunostimulatory factors in RAW264.7 cells. TLR2 and TLR4 blocked the production of immunostimulatory factors by WCG-S in RAW264.7 cells. In addition, the inhibition of MAPK and NF-κB signalling pathway reduced WCG-S-mediated production of immunostimulatory factors and the activation of MAPK and NF-κB signalling pathway by WCG-S was suppressed by the inhibition of TLR2/4. These findings indicate that WCG-R and WCG-S may be considered to have anti-obesity and immunostimulatory activity.
中文翻译:
木栽培人参在小鼠巨噬细胞、RAW264.7 细胞中发挥免疫刺激活性,在小鼠脂肪细胞、3T3-l1 细胞中发挥抗肥胖活性
摘要
我们在体外评估木栽培人参的抗肥胖和免疫刺激活性。木栽培人参的根 (WCG-R) 和茎 (WCG-S) 降低了 3T3-L1 细胞中的脂质积累和 TG 含量。WCG-R和WCG-S抑制与成熟脂肪细胞脂质积累相关的CEBPα、PPARγ、perilipin-1、脂联素和FABP4等蛋白质表达。此外,WCG-R 和 WCG-S 在从前脂肪细胞到成熟脂肪细胞的分化过程中降低了 CEBPα 和 PPARγ。WCG-S 增加了 RAW264.7 细胞中免疫刺激因子的产生。TLR2 和 TLR4 阻止 WCG-S 在 RAW264.7 细胞中产生免疫刺激因子。此外,MAPK 和 NF-κB 信号通路的抑制减少了 WCG-S 介导的免疫刺激因子的产生,WCG-S 对 MAPK 和 NF-κB 信号通路的激活被 TLR2/4 的抑制所抑制。这些发现表明 WCG-R 和 WCG-S 可能被认为具有抗肥胖和免疫刺激活性。