A new cost-effective NHS functionalized polyoxazoline (POx) loaded polymer with strong hemostatic properties has been developed. In this study, we investigate POx loaded hemostatic patches regarding hemostatic efficacy, local inflammatory reaction and wound-healing, as compared to the non-POx treated blanks and commercially available hemostatic products. Hundred and ten rats divided into 11 groups of 10 animals underwent partial liver lobe resection. Eight groups received experimental patches, two groups commercially available hemostatic patches (TachoSil® and Veriset™, positive controls), one group with gauzes (negative control). Each animal received twice a patch with a size 1.5 × 2.5 cm, on each partially resected lobe. Primary endpoint was time to hemostasis (TTH). The rats were sacrificed at different time points (1, 3, or 7 days) to measure local inflammatory response and early wound healing. Of the POx loaded patches, GFC NHS-POx (TTH 20.4 s, p = .019) and GFC-NHS-POx1.5 (TTH 0.0 s, p = .003) showed significantly faster TTH compared to TachoSil® (TTH 95.4 s), and were comparable to Veriset™ (TTH 17.0 s). Three patches, GFC-NHS-POx 1.5 (TTH 0.0 s, p = .016), ORC NHS-POx:NU-POx (TTH 91.4 s, p = .033), and ORC-PLGA NHS-POx:NU-POx (TTH 105.6 s, p = .04) had a lower TTH compared to their own blank carrier (TTH 74.9, 157.8, and 195.7 s, respectively). With regard to biocompatibility, all POx loaded patches showed results comparable to TachoSil® and Veriset™. NHS-POx-loaded hemostatic patch demonstrate fast and effective hemostasis, comparable or better than commercially available hemostatic patches, with similar early biocompatibility.

中文翻译：

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用于实验性肝切除术中止血的新型聚恶唑啉负载贴片

已经开发出一种新的具有成本效益的具有强止血性能的 NHS 功能化聚恶唑啉 (POx) 负载聚合物。在这项研究中，我们研究了负载 POx 的止血贴片的止血效果、局部炎症反应和伤口愈合，与未经 POx 处理的空白和市售止血产品相比。10只11组10只大鼠接受部分肝叶切除术。八组接受实验性贴片，两组市售止血贴片（TachoSil® 和 Veriset™，阳性对照），一组使用纱布（阴性对照）。每只动物在每个部分切除的肺叶上接受两次大小为 1.5 × 2.5 cm 的贴片。主要终点是止血时间（TTH）。在不同时间点处死大鼠（1、3、或 7 天）以测量局部炎症反应和早期伤口愈合。在 POx 加载补丁中，GFC NHS-POx (TTH 20.4 s,p  = .019) 和 GFC-NHS-POx1.5 (TTH 0.0 s, p  = .003) 与 TachoSil® (TTH 95.4 s) 相比显示出明显更快的 TTH，并且与 Veriset™ (TTH 17.0 s) 相当。三个贴片，GFC-NHS-POx 1.5 (TTH 0.0 s, p  = .016), ORC NHS-POx:NU-POx (TTH 91.4 s, p  = .033) 和 ORC-PLGA NHS-POx:NU-POx (TTH 105.6 s, p  = .04) 与他们自己的空白载体相比具有较低的 TTH（TTH 分别为 74.9、157.8 和 195.7 s）。在生物相容性方面，所有负载 POx 的贴片都显示出与 TachoSil® 和 Veriset™ 相当的结果。加载 NHS-POx 的止血贴片显示出快速有效的止血效果，与市售的止血贴片相当或更好，具有相似的早期生物相容性。