A novel Gram-positive, facultatively anaerobic, rod-shaped, nonspore forming, nonmotile organism was isolated from a Japanese serow oral cavity. Designated strain MAS-1^T, it is most closely related to Actinomyces bowdenii DSM 15435^T, with which it shares 98.07% sequence homology in the 16S ribosomal RNA gene. The primarily detected cellular fatty acids in strain MAS-1^T were C_16:0 and C_18:1w9c. The predominant respiratory quinone was MK-9 (H₄). The major polar lipids were phosphatidylcholines, phosphatidylinositols, and glycophospholipids. The genomic DNA GC content of the isolate was 71.3 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between MAS-1^T and its related species were 23.5%–39.5% and 82.11%–91.01%, respectively, which were below the threshold (70% and 95%, respectively) for species delineation, indicating that strain MAS-1^T represents a novel species. Strain MAS-1^T can be differentiated from A. bowdenii by their reactions to naphthol-AS-BI-phosphohydrolase, α-galactosidase, β-galactosidase, and N-acetyl-β-glucosaminidase, as well as differing acid production from glycogen. Based on the results of genotypic, phenotypic, and biochemical analyses, herein it is proposed that the identified bacteria can be classified as a novel species, Actinomyces capricornis sp. nov., strain MAS-1^T (=JCM 34236^T = DSM 111732^T).

中文翻译：

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山羊放线菌 sp. 11 月，从日本赛罗的口腔中分离出来

一种新型革兰氏阳性、兼性厌氧、杆状、不形成孢子、不运动的生物体是从日本血清的口腔中分离出来的。指定菌株 MAS-1 ^T，它与鲍登放线菌DSM 15435 ^{T 的}亲缘关系最密切，与它在 16S 核糖体 RNA 基因中有 98.07% 的序列同源性。在菌株 MAS-1 ^T中主要检测到的细胞脂肪酸是 C _16:0和 C _18:1 w9c。主要的呼吸醌是 MK-9 (H ₄）。主要的极性脂质是磷脂酰胆碱、磷脂酰肌醇和糖磷脂。分离物的基因组 DNA GC 含量为 71.3 mol%。MAS-1 ^T与其相关物种之间的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 23.5%–39.5% 和 82.11%–91.01%，低于阈值（分别为 70% 和 95%）物种划分，表明菌株 MAS-1 ^T代表一个新物种。菌株 MAS-1 ^T可以通过它们对萘酚-AS - BI-磷酸水解酶、α-半乳糖苷酶、β-半乳糖苷酶和N的反应与鲍登曲霉区分开来-乙酰-β-氨基葡萄糖苷酶，以及由糖原产生的不同酸。基于基因型、表型和生化分析结果，本文提出鉴定的细菌可归类为新种，山羊放线菌。nov., 菌株 MAS-1 ^T (=JCM 34236 ^T  = DSM 111732 ^T )。