Inhibitory properties of polyphenols in Malus “Winter Red” crabapple fruit on α-glucosidase and α-amylase using improved methods,Journal of Food Biochemistry

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Inhibitory properties of polyphenols in Malus “Winter Red” crabapple fruit on α-glucosidase and α-amylase using improved methods
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-09-18 , DOI: 10.1111/jfbc.13942
Zhengcao Xiao _{1,

2,

3,

4} , Ruijia Yang ₁ , Haojie Wang ₁ , Xiaohui Cui ₁ , Yunyuan Zhang ₅ , Yahong Yuan ₆ , Tianli Yue _{2,

3,

4} , Pengmin Li ₁

Affiliation

To explore the inhibitory activity of polyphenols on α-glucosidase and α-amylase, 16 polyphenols were isolated, identified, and quantified in an edible Malus “Winter Red” crabapple fruit. The limitations of two traditional methods for α-glucosidase and α-amylase activity assay in vitro were assayed. An improved method based on an HPLC assay for α-glucosidase and a colorimetric method coupled with a custom-made mini-column for α-amylase were established. Compared with positive controls, acarbose and miglito, most polyphenols, especially the four aglycones (cyanidin, quercetin, phloretin, and 3-hydroxyphloretin) showed higher inhibition rates on α-glucosidase. None of the polyphenols showed higher inhibition rates on α-amylase than acarbose, but most, especially the four aglycones, showed higher inhibition rates on α-amylase than miglito. The Malus Winter Red fruit has great potential for postprandial blood glucose management as a potential diet therapy for diabetic patients.

中文翻译：

使用改进方法研究苹果“冬红”海棠果实中多酚对α-葡萄糖苷酶和α-淀粉酶的抑制特性

为了探索多酚对 α-葡萄糖苷酶和 α-淀粉酶的抑制活性，在可食用的苹果中分离、鉴定和定量了 16 种多酚“冬红”海棠果实。测定了体外 α-葡萄糖苷酶和 α-淀粉酶活性测定的两种传统方法的局限性。建立了一种基于 HPLC 测定 α-葡萄糖苷酶的改进方法和一种与定制的 α-淀粉酶微型柱相结合的比色法。与阳性对照、阿卡波糖和米格列托相比，大多数多酚，尤其是四种苷元（花青素、槲皮素、根皮素和3-羟基根皮素）对α-葡萄糖苷酶的抑制率更高。没有一种多酚对 α-淀粉酶的抑制率高于阿卡波糖，但大多数，尤其是四种苷元，对 α-淀粉酶的抑制率高于 miglito。在海棠冬季红果作为糖尿病患者的潜在饮食疗法，在餐后血糖管理方面具有巨大潜力。

更新日期：2021-10-06

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