In this study, a magnetic yolk-shell structured metal-organic framework material (Fe3O4@YS-UiO-66-NH2) is prepared by the directional etching of Co2+/peroxymonosulfate and in situ magnetization. The characteristic properties of the material were investigated by using field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometer, Brunauer-Emmett-Teller, and contact angle test. The Fe3O4@YS-UiO-66-NH2 shows the advantages of large surface area, good magnetic property, and satisfactory stability, as well as giving high affinity to alkaloids (ALs) via hydrophilic interaction, hydrogen bonding, and π-π interaction. The results of static adsorption experiment indicate that the Fe3O4@YS-UiO-66-NH2 possesses high adsorption capacity towards ALs and the adsorption behaviors are fitted with Langmuir adsorption isotherm model. Furthermore, a magnetic solid-phase extraction using Fe3O4@YS-UiO-66-NH2 and HPLC method was developed for the analysis of ALs in spiked samples with the recovery of 89.6-100.8%. In addition, the proposed method was successfully applied in the pharmacokinetics study of berberine, coptisine, and palmatine in the rat. In short, the developed method might be used for high-efficient recognition and determination of ALs in plasma sample, which would also provide a new way to fabricate magnetic functionalized metal-organic framework in separation science.

中文翻译：

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磁性蛋黄壳结构金属有机骨架材料的制备及其在生物碱药代动力学研究中的应用

本研究通过Co2+/过氧单硫酸盐的定向刻蚀和原位磁化制备了一种磁性蛋黄壳结构的金属有机骨架材料（Fe3O4@YS-UiO-66-NH2）。采用场发射扫描电子显微镜、透射电子显微镜、能量色散 X 射线光谱、X 射线粉末衍射、傅里叶变换红外光谱、振动样品磁力计、Brunauer-Emmett-Teller 和接触角测试。Fe3O4@YS-UiO-66-NH2具有比表面积大、磁性好、稳定性好等优点，并通过亲水作用、氢键作用和π-π作用对生物碱（ALs）具有高亲和力。静态吸附实验结果表明，Fe3O4@YS-UiO-66-NH2对ALs具有较高的吸附能力，吸附行为符合Langmuir吸附等温线模型。此外，还开发了一种使用 Fe3O4@YS-UiO-66-NH2 和 HPLC 的磁性固相萃取方法，用于分析加标样品中的 ALs，回收率为 89.6-100.8%。此外，该方法成功地应用于黄连素、黄连碱和巴马汀在大鼠体内的药代动力学研究。总之，所开发的方法可用于血浆样品中ALs的高效识别和测定，也将为分离科学中制备磁性功能化金属有机骨架提供新的途径。