Two aphid-transmitted RNA viruses, broad bean wilt virus 2 (BBWV2) and cucumber mosaic virus (CMV), are the most prevalent viruses in Korean pepper fields and cause chronic damage in pepper production. In this study, we employed a screening system for pathotype-specific resistance of pepper germplasm to BBWV2 and CMV by utilizing infectious cDNA clones of different pathotypes of the viruses (two BBWV2 strains and three CMV strains). We first examined pathogenic characteristics of the BBWV2 and CMV strains in various plant species and their phylogenetic positions in the virus population structures. We then screened 34 commercial pepper cultivars and seven accessions for resistance. While 21 pepper cultivars were resistant to CMV Fny strain, only two cultivars were resistant to CMV P1 strain. We also found only one cultivar partially resistant to BBWV2 RP1 strain. However, all tested commercial pepper cultivars were susceptible to the resistance-breaking CMV strain GTN (CMV-GTN) and BBWV2 severe strain PAP1 (BBWV2-PAP1), suggesting that breeding new cultivars resistant to these virus strains is necessary. Fortunately, we identified several pepper accessions that were resistant or partially resistant to CMV-GTN and one symptomless accession despite systemic infection with BBWV2-PAP1. These genetic resources will be useful in pepper breeding programs to deploy resistance to BBWV2 and CMV.

中文翻译：

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辣椒（Capsicum annuum L.）对蚕豆枯萎病毒2型和黄瓜花叶病毒的病原型特异性抗性筛选

两种由蚜虫传播的 RNA 病毒，蚕豆枯萎病毒 2 (BBWV2) 和黄瓜花叶病毒 (CMV)，是韩国辣椒田中最流行的病毒，会对辣椒生产造成慢性损害。在本研究中，我们利用不同致病型病毒（两种 BBWV2 毒株和三种 CMV 毒株）的感染性 cDNA 克隆，采用了辣椒种质对 BBWV2 和 CMV 的致病型特异性抗性筛选系统。我们首先检查了 BBWV2 和 CMV 毒株在各种植物物种中的致病特征及其在病毒种群结构中的系统发育位置。然后，我们筛选了 34 个商业辣椒品种和 7 个品种的抗性。21个辣椒品种对CMV Fny株具有抗性，而只有两个品种对CMV P1株具有抗性。我们还发现只有一个品种对 BBWV2 RP1 菌株具有部分抗性。然而，所有测试的商业辣椒品种都对抗性破坏 CMV 毒株 GTN (CMV-GTN) 和 BBWV2 重毒株 PAP1 (BBWV2-PAP1) 敏感，这表明培育对这些病毒株具有抗性的新品种是必要的。幸运的是，我们发现了几种对 CMV-GTN 具有抗性或部分抗性的辣椒种质，以及一种无症状的种质，尽管全身感染了 BBWV2-PAP1。这些遗传资源将有助于辣椒育种计划部署对 BBWV2 和 CMV 的抗性。这表明培育对这些病毒株具有抗性的新品种是必要的。幸运的是，我们发现了几种对 CMV-GTN 具有抗性或部分抗性的辣椒种质，以及一种无症状的种质，尽管全身感染了 BBWV2-PAP1。这些遗传资源将有助于辣椒育种计划部署对 BBWV2 和 CMV 的抗性。这表明培育对这些病毒株具有抗性的新品种是必要的。幸运的是，我们发现了几种对 CMV-GTN 具有抗性或部分抗性的辣椒种质，以及一种无症状的种质，尽管全身感染了 BBWV2-PAP1。这些遗传资源将有助于辣椒育种计划部署对 BBWV2 和 CMV 的抗性。