Gray leaf spot (GLS), caused by different species of Cercospora, is a fungal, non-soil-borne disease that causes serious reductions in maize yield worldwide. The identification of major quantitative trait loci (QTLs) for GLS resistance in maize is essential for developing marker-assisted selection strategies in maize breeding. Previous research found a significant difference (P < 0.01) in GLS resistance between T32 (highly resistant) and J51 (highly susceptible) genotypes of maize. Initial QTL analysis was conducted in an F_{2 : 3} population of 189 individuals utilizing genetic maps that were constructed using 181 simple sequence repeat (SSR) markers. One QTL (qGLS8) was detected, defined by the markers umc1130 and umc2354 in three environments. The qGLS8 QTL detected in the initial analysis was located in a 51.96-Mb genomic region of chromosome 8 and explained 7.89–14.71% of the phenotypic variation in GLS resistance in different environments. We also developed a near isogenic line (NIL) BC₃F₂ population with 1,468 individuals and a BC₃F₂-Micro population with 180 individuals for fine mapping. High-resolution genetic and physical maps were constructed using six newly developed SSRs. The QTL-qGLS8 was narrowed down to a 124-kb region flanked by the markers ym20 and ym51 and explained up to 17.46% of the phenotypic variation in GLS resistance. The QTL-qGLS8 contained seven candidate genes, such as an MYB-related transcription factor 24 and a C₃H transcription factor 347), and long intergenic non-coding RNAs (lincRNAs). The present study aimed to provide a foundation for the identification of candidate genes for GLS resistance in maize.

灰叶斑病 (GLS)，由不同种类的 尾孢, 是一种真菌性、非土壤传播的疾病，会导致全球玉米产量严重下降。鉴定玉米 GLS 抗性的主要数量性状位点 (QTL) 对于开发玉米育种中的标记辅助选择策略至关重要。先前的研究发现了显着差异（磷< 0.01) 玉米 T32（高抗性）和 J51（高感性）基因型之间的 GLS 抗性。利用使用181个简单序列重复(SSR)标记构建的遗传图在189个个体的F _{2 : 3}群体中进行初始QTL分析。一个 QTL (qGLS8) 被检测到，由三个环境中的标记 umc1130 和 umc2354 定义。这qGLS8在初始分析中检测到的 QTL 位于 8 号染色体的 51.96-Mb 基因组区域，并解释了不同环境中 GLS 抗性表型变异的 7.89-14.71%。我们还开发了一个具有 1,468 个个体的近等基因系 (NIL) BC ₃ F ₂种群和一个具有 180 个个体的 BC ₃ F ₂ -Micro 种群，用于精细定位。高分辨率遗传和物理图谱是使用六个新开发的 SSR 构建的。QTL-qGLS8被缩小到 124-kb 区域，两侧是标记 ym20 和 ym51，并解释了高达 17.46% 的 GLS 抗性表型变异。QTL-qGLS8 包含七个候选基因，例如一个 MYB 相关转录因子 24 和一个 C₃H 转录因子 347) 和长基因间非编码 RNA (lincRNA)。本研究旨在为鉴定玉米GLS抗性候选基因提供基础。