Nuclear factor Y (NF-Y) is a ubiquitous transcription factor in eukaryotes, which is composed of three subunits (NF-YA, NF-YB, and NF-YC). NF-Y has been identified as a key regulator of multiple pathways in plants. Although the NF-Y gene family has been identified in many plants, it has not been reported in potato (Solanum tuberosum). In the present study, a total of 41 NF-Y proteins in potato (StNF-Ys) were identified, including 10 StNF-YA, 22 StNF-YB, and nine StNF-YC subunits, and their distribution on chromosomes, gene structure, and conserved motif was analyzed. A synteny analysis indicated that 14 and 38 pairs of StNF-Y genes were orthologous to Arabidopsis and tomato (Solanum lycopersicum), respectively, and these gene pairs evolved under strong purifying selection. In addition, we analyzed the expression profiles of NF-Y genes in different tissues of double haploid (DM) potato, as well as under abiotic stresses and hormone treatments by RNA-seq downloaded from the Potato Genome Sequencing Consortium (PGSC) database. Furthermore, we performed RNA-seq on white, red, and purple tuber skin and flesh of three potato cultivars at the tuber maturation stage to identify genes that might be involved in anthocyanin biosynthesis. These results provide valuable information for improved understanding of StNF-Y gene family and further functional analysis of StNF-Y genes in fruit development, abiotic stress tolerance, and anthocyanin biosynthesis in potato.

核因子 Y (NF-Y) 是真核生物中普遍存在的转录因子，由三个亚基（NF-YA、NF-YB 和 NF-YC）组成。NF-Y 已被确定为植物中多种途径的关键调节剂。虽然NF-Y基因家族已经在许多植物中被鉴定出来，但在马铃薯中尚未见报道。马铃薯）。本研究共鉴定马铃薯中 41 个 NF-Y 蛋白（StNF-Ys），包括 10 个 StNF-YA、22 个 StNF-YB 和 9 个 StNF-YC 亚基，及其在染色体、基因结构、并对保守基序进行了分析。同线性分析表明，14 和 38 对 StNF-Y 基因与拟南芥 和番茄（茄)，并且这些基因对在强纯化选择下进化。此外，我们通过从马铃薯基因组测序联盟 (PGSC) 数据库下载的 RNA-seq 分析了 NF-Y 基因在双单倍体 (DM) 马铃薯不同组织以及非生物胁迫和激素处理下的表达谱。此外，我们在块茎成熟阶段对三个马铃薯品种的白色、红色和紫色块茎皮和肉进行了 RNA-seq，以确定可能参与花青素生物合成的基因。这些结果为提高对 StNF-Y 基因家族的理解以及 StNF-Y 基因在马铃薯果实发育、非生物胁迫耐受性和花青素生物合成中的进一步功能分析提供了有价值的信息。