Introduction: Multiple sclerosis (MS) is known to be a multifactorial disorder. Numerous observational studies have suggested the implication of multiple genetic and environmental factors in the pathogenesis of MS. The aim of this work was to evaluate expression of the microRNA-22 (miRNA-22) level, in relation to vitamin D (VD) and VD receptor (VDR) levels in patients with MS during remission state. Methods: This case-control study was conducted in 50 patients with clinically definite MS and 50 age- and sex-matched healthy controls. miRNA-22 expression was assessed in both MS patients and controls using quantitative RT-PCR. The serum level of VD and VDR was assessed in both MS patients and controls using ELISA techniques. Results: The miRNA-22 level was significantly downregulated in MS patients in comparison to controls (p value #x3c;0.001). MS patients had also significantly lower VD and VDR levels in comparison to controls (p value #x3c;0.001 and #x3c;0.001, respectively). Patients with secondary progressive MS (SPMS) have a significantly higher miRNA-22 level than patients with relapsing remitting MS (RRMS) (p value = 0.042). There was a statistically significant positive correlation between the miRNA-22 level and EDSS (p value = 0.033). There was also a statistically significant positive correlation between the miRNA-22 level and VDR level (p value = 0.002). Conclusion: The miRNA-22 level was significantly downregulated in MS patients, but it had a positive correlation with disability status. Patients with SPMS have a significantly higher miRNA-22 level than patients with RRMS. VD and VDR levels were significantly lower in MS patients than controls. The miRNA-22 level was positively correlated with the VDR level.
Neuroimmunomodulation

中文翻译：

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多发性硬化症患者的 MicroRNA-22 水平及其与维生素 D 和维生素 D 受体水平的关系

简介：众所周知，多发性硬化症 (MS) 是一种多因素疾病。大量观察性研究表明，多种遗传和环境因素在 MS 发病机制中的意义。这项工作的目的是评估缓解状态期间 MS 患者中 microRNA-22 (miRNA-22) 水平与维生素 D (VD) 和 VD 受体 (VDR) 水平的关系。方法：这项病例对照研究在 50 名临床确诊 MS 患者和 50 名年龄和性别匹配的健康对照中进行。使用定量 RT-PCR 在 MS 患者和对照中评估 miRNA-22 表达。使用 ELISA 技术在 MS 患者和对照中评估 VD 和 VDR 的血清水平。结果：与对照组相比，MS 患者的 miRNA-22 水平显着下调（p值 #x3c；0.001）。与对照组相比，MS 患者的 VD 和 VDR 水平也显着降低（p值分别为 #x3c；0.001 和 #x3c；0.001）。继发进展型 MS (SPMS) 患者的 miRNA-22 水平显着高于复发缓解型 MS (RRMS) 患者（p值 = 0.042）。miRNA-22水平与EDSS呈统计学显着正相关（p值=0.033）。miRNA-22水平与VDR水平之间也存在统计学上显着的正相关（p值= 0.002）。结论：MS患者的miRNA-22水平显着下调，但与残疾状况呈正相关。SPMS 患者的 miRNA-22 水平显着高于 RRMS 患者。MS 患者的 VD 和 VDR 水平显着低于对照组。miRNA-22水平与VDR水平呈正相关。
神经免疫调节