A novel microfluidic optical cell is presented that enables simultaneous measurement of both light absorbance and fluorescence on microlitre volumes of fluid. The chip design is based on an inlaid fabrication technique using clear and opaque poly(methyl methacrylate) or PMMA to create a 20.2 mm long optical cell. The inlaid approach allows fluid interrogation with minimal interference from external light over centimeter long path lengths. The performance of the optical cell is evaluated using a stable fluorescent dye: rhodamine B. Excellent linear relationships (R² > 0.99) are found for both absorbance and fluorescence over a 0.1–10 µM concentration range. Furthermore, the molar attenuation spectrum is accurately measured over the range 460–550 nm. The approach presented here is applicable to numerous colorimetric- or fluorescence-based assays and presents an important step in the development of multipurpose lab-on-chip sensors.

中文翻译：

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使用镶嵌微流体方法同时进行吸光度和荧光测量

提出了一种新型微流体光学单元，可以同时测量微升体积的流体的光吸收和荧光。芯片设计基于镶嵌制造技术，使用透明和不透明的聚（甲基丙烯酸甲酯）或 PMMA 来创建 20.2 毫米长的光学单元。镶嵌方法允许在厘米长的路径长度上以最小的外部光干扰进行流体询问。使用稳定的荧光染料：罗丹明 B 评估光学池的性能。出色的线性关系 (R ²> 0.99) 在 0.1–10 µM 浓度范围内的吸光度和荧光。此外，摩尔衰减光谱在 460–550 nm 范围内被准确测量。此处介绍的方法适用于许多基于比色或荧光的检测方法，是开发多用途芯片实验室传感器的重要一步。