The study evaluated the dose–response relationship of two commercial microbial biocontrol agents, Bacillus subtilis and Gliocladium catenulatum, against Botrytis cinerea both in vitro and in vivo. Inoculum doses, formulation, temperature and foliar leaf part all affected the control achieved by the two BCAs. In vitro competition assays on modified PDA plates tested a range of BCA doses (log₁₀ 3–10 CFUs or spores/droplet) at 4, 10 and 20 °C on the development of B. cinerea colonies. The dose–response relationship was influenced by both the BCA formulation and temperature. In vivo studies on lettuce plants in semi-commercial greenhouses examined the BCA dose (log₁₀ 5–9 CFUs or spores/mL) for controlling B. cinerea with a high inoculum (log₁₀ 6 spores/mL). Leaf disc assays showed that the dose–response relationship was influenced by the leaf parts sampled. These results suggest that the dose–response relationship between a BCA and specific pathogen will be significantly influenced by environmental conditions, formulation and plant phyllosplane tissue.

中文翻译：

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对两种商业生物防治剂在与不同非生物因素相关的体外和体内条件下对 B. cinerea 的功效的批判性评价

该研究在体外和体内评估了两种商业微生物生物防治剂枯草芽孢杆菌和Gliocladium catenulatum对灰葡萄孢菌的剂量-反应关系。接种剂量、配方、温度和叶部分都影响了两种 BCA 实现的控制。在4、10 和 20 °C 下，改良 PDA 平板上的体外竞争测定测试了一系列 BCA 剂量（log ₁₀ 3–10 CFU 或孢子/液滴）对B. cinerea菌落发育的影响。剂量-反应关系受 BCA 配方和温度的影响。在半商业温室中对生菜植物进行的体内研究检查了 BCA 剂量（log ₁₀5–9 CFU 或孢子/mL），用于控制高接种量（log ₁₀ 6 孢子/mL）的灰霉病。叶盘测定表明剂量-反应关系受采样的叶部分的影响。这些结果表明，BCA 与特定病原体之间的剂量-反应关系将受到环境条件、配方和植物叶面组织的显着影响。