ABSTRACT

Purpose

During the COVID-19 pandemic, healthcare workers (HCWs) are at a considerable risk of being infected with SARS-CoV-2; among them, HCWs from ophthalmology departments are more prone to develop severe symptoms. In Mexico City, the prevalence of SARS-CoV-2 infection among HCWs is 30%. The present work aims to describe the seroprevalence among HCWs at an Ophthalmological Reference Centre in Mexico City.

Methods

A self-report questionnaire, RT-PCR test and detection of serum IgG/IgM antibodies against SARS-CoV-2 were performed among HCWs at the Institute of Ophthalmology “Conde de Valenciana”.

Results

A total of 169 HCWs participated in the study. None of the participants declared severe symptoms, and only 15% showed three or more symptoms. The results showed that 32% of the participants were RT-PCR⁺ (54/169), and 20% (35/169) presented IgG antibodies against SARS-CoV-2. Thirteen percent of the RT-PCR⁺ subjects were IgG positive, and 7.6% of the RT-PCR⁻ participants were IgG positive. The presence of three or more symptoms correlated with the presence of IgG antibodies, as well as Ct values of < 32 (p < 0,05).

Conclusion

Most of the HCW cohort showed mild symptoms, and 69% of the RT-PCR⁺ participants did not show IgG antibodies against SARS-CoV-2. Seroprevalence was significantly associated with the presentation of COVID-19-associated symptoms.

中文翻译：

---

一项横断面研究，眼科参考中心医护人员中的 SARS-CoV-2 血清阳性率

摘要

目的

在 COVID-19 大流行期间，医护人员 (HCW) 感染 SARS-CoV-2 的风险很大；其中，眼科医护人员更容易出现严重症状。在墨西哥城，医护人员中 SARS-CoV-2 的感染率为 30%。目前的工作旨在描述墨西哥城眼科参考中心 HCW 的血清阳性率。

方法

在“Conde de Valenciana”眼科研究所的医护人员中进行了自我报告问卷、RT-PCR 测试和血清 IgG/IgM 抗体检测，以检测 SARS-CoV-2。

结果

共有 169 名医护人员参与了这项研究。没有参与者宣布出现严重症状，只有 15% 的参与者表现出三种或更多症状。结果显示，32% 的参与者为 RT-PCR ⁺ (54/169)，20% (35/169) 的参与者呈现针对 SARS-CoV-2 的 IgG 抗体。13% 的 RT-PCR ⁺受试者为 IgG 阳性，7​​.6% 的 RT-PCR ^-受试者为IgG 阳性。三个或更多症状的存在与 IgG 抗体的存在相关，以及 Ct 值 < 32 ( p < 0,05)。

结论

大多数 HCW 队列表现出轻微症状，69% 的 RT-PCR ⁺参与者没有显示针对 SARS-CoV-2 的 IgG 抗体。血清阳性率与 COVID-19 相关症状的表现显着相关。