A novel closed-tube loop-mediated isothermal amplification (LAMP) was developed to detect goat milk adulterated with cow milk. The reaction results of the LAMP assay developed, identified using both visual observation and agarose gel electrophoresis, were completely consistent, indicating that visual observation was suitable and efficient to judge the final LAMP results. The specificity of LAMP assay was evaluated using DNA of related species namely goat, pork, chicken, duck, dog, which revealed only a specific amplification of cow. The sensitivity of LAMP assay was as low as 10 fg. The limit of detection (LOD) of LAMP assay reached 0.01%, 10 times higher than PCR assay. In addition, this assay successfully amplified and identified 0.01% of cow commercial milk products in goat milk. Therefore, our results provided a simple, specific, sensitive, rapid, and cost-effective method to detect goat milk adulteration.

开发了一种新型闭管环介导的等温扩增 (LAMP) 来检测掺有牛奶的山羊奶。通过目视观察和琼脂糖凝胶电泳确定的 LAMP 测定的反应结果完全一致，表明目视观察是判断最终 LAMP 结果的合适和有效的方法。LAMP 检测的特异性是使用相关物种的 DNA 评估的，即山羊、猪肉、鸡、鸭、狗，这仅揭示了牛的特异性扩增。LAMP 检测的灵敏度低至 10 fg。LAMP 检测的检测限 (LOD) 达到 0.01%，比 PCR 检测高 10 倍。此外，该检测成功地扩增并鉴定了山羊奶中 0.01% 的牛市售奶制品。因此，我们的结果提供了一种简单、特异、灵敏、