In the current work, a fluorescence-based method with a point-of-use colorimetric sensing system was developed to test the quality of the milk in real-time using fluorophores. The fluorescence intensities of fluorophores were optimised to predict the pH of the milk samples using colour sensor device (CSD) and were cross-reference using a fibre optic spectrophotometer (FOS). The developed CSD and FOS measured the pH and adulteration in a linear range of 4–9 pH units and 0–70 mm urea in milk with a quick response time of 30 s and 5 min. The interday variability for pH sensing by the CSD and FOS was evaluated and expressed as a percentage relative standard deviation (%RSD) which was found to be 1.89 for CSD and 4.72 for FOS. The results indicate that CSD and FOS can be suitable tools for spoilage and urea adulteration detection in milk samples.

在当前的工作中，开发了一种具有使用点比色传感系统的基于荧光的方法，以使用荧光团实时测试牛奶的质量。使用颜色传感器装置 (CSD) 优化荧光团的荧光强度以预测牛奶样品的 pH 值，并使用光纤分光光度计 (FOS) 进行交叉参考。开发的 CSD 和 FOS 在 4–9 pH 单位和 0–70 m m的线性范围内测量 pH 和掺假牛奶中尿素的快速响应时间为 30 秒和 5 分钟。CSD 和 FOS 检测 pH 值的日间变异性被评估并表示为百分比相对标准偏差 (%RSD)，发现 CSD 为 1.89，FOS 为 4.72。结果表明，CSD 和 FOS 可作为检测牛奶样品中腐败和尿素掺假的合适工具。