Antimicrobial peptide activity in asymmetric bacterial membrane mimics,Faraday Discussions

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Antimicrobial peptide activity in asymmetric bacterial membrane mimics
Faraday Discussions ( IF 3.3 ) Pub Date : 2021-07-21 , DOI: 10.1039/d1fd00039j
Lisa Marx _{1,

2} , Moritz P K Frewein _{1,

2,

3} , Enrico F Semeraro _{1,

2} , Gerald N Rechberger _{1,

2} , Karl Lohner _{1,

2} , Lionel Porcar ₃ , Georg Pabst _{1,

2}

Affiliation

We report on the response of asymmetric lipid membranes composed of palmitoyl oleoyl phosphatidylethanolamine and palmitoyl oleoyl phosphatidylglycerol, to interactions with the frog peptides L18W-PGLa and magainin 2 (MG2a), as well as the lactoferricin derivative LF11-215. In particular we determined the peptide-induced lipid flip-flop, as well as membrane partitioning of L18W-PGLa and LF11-215, and vesicle dye-leakage induced by L18W-PGLa. The ability of L18W-PGLa and MG2a to translocate through the membrane appears to correlate with the observed lipid flip-flop, which occurred at the fastest rate for L18W-PGLa. The higher structural flexibility of LF11-215 in turn allows this peptide to insert into the bilayers without detectable changes of membrane asymmetry. The increased vulnerability of asymmetric membranes to L18W-PGLa in terms of permeability, appears to be a consequence of tension differences between the compositionally distinct leaflets, but not due to increased peptide partitioning.

中文翻译：

不对称细菌膜模拟物中的抗菌肽活性

我们报告了由棕榈酰油酰磷脂酰乙醇胺和棕榈酰油酰磷脂酰甘油组成的不对称脂质膜对青蛙肽 L18W-PGLa 和 Magainin 2 (MG2a) 以及乳铁蛋白衍生物 LF11-215 相互作用的响应。特别是，我们确定了肽诱导的脂质翻转，以及 L18W-PGLa 和 LF11-215 的膜分配，以及 L18W-PGLa 诱导的囊泡染料渗漏。 L18W-PGLa 和 MG2a 穿过膜易位的能力似乎与观察到的脂质翻转相关，L18W-PGLa 发生的脂质翻转速度最快。 LF11-215 较高的结构灵活性反过来又允许该肽插入双层，而不会发生可检测到的膜不对称性变化。就渗透性而言，不对称膜对 L18W-PGLa 的脆弱性增加似乎是成分不同的小叶之间张力差异的结果，而不是由于肽分配的增加。

更新日期：2021-09-17

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