Light management is important for improving algae cultivation, specifically by enhancing the productivity of biomass and valued bioproducts. In this study, we present evidence that alternating blue and red-orange light can improve the algal growth kinetics and lipid production in a photobioreactor. Blue (430–445, 460–470 nm) and red-orange light (580–660 nm) from a LED were set at the light saturation point (B: 65 μmol/m²s; RO: 155 μmol/m²s) and alternated for the cultivation of the green alga Chlamydomonas reinhardtii. Growth kinetics, lipid, carbohydrate, and protein content were measured as a function of alternating illumination time. Results reveal that the first illumination light and illumination time had a significant impact on the growth kinetics and nutrient composition. When the red-orange light illumination was used at the beginning of cultivation (RO/B alternation), the biomass concentration and productivity increased 8% and 18% on average, respectively; lipid mass fraction and concentration increased 21–27% and 24–26% when 0.25–0.50 h per day of blue light illumination was used; no significant change of carbohydrate and protein content were observed. Relative to blue light alone, the improvement of growth kinetics, lipid mass fraction and concentration, and the carbohydrate concentration was significant. Under B/RO alternation (when the blue light was used first), on average, the protein content was significantly higher than RO/B alternation.

光照管理对于改善藻类培养很重要，特别是通过提高生物质和有价值的生物产品的生产力。在这项研究中，我们提出证据表明交替的蓝光和红橙色光可以改善光生物反应器中的藻类生长动力学和脂质生产。来自 LED 的蓝色 (430–445, 460–470 nm) 和红橙色光 (580–660 nm) 设置在光饱和点 (B: 65 μmol/m ² s; RO: 155 μmol/m ² s ) 并交替培养绿藻莱茵衣藻. 生长动力学、脂质、碳水化合物和蛋白质含量被测量为交替光照时间的函数。结果表明，首次光照和光照时间对生长动力学和营养成分有显着影响。栽培开始时使用红橙色光照明（RO/B交替），生物量浓度和生产力分别平均提高8%和18%；当每天使用 0.25-0.50 小时的蓝光照明时，脂质质量分数和浓度增加了 21-27% 和 24-26%；没有观察到碳水化合物和蛋白质含量的显着变化。相对于单独的蓝光，生长动力学、脂质质量分数和浓度以及碳水化合物浓度的改善是显着的。