Scaling-up techniques in temporary immersion systems are an alternative for commercial micropropagation. In vitro propagation of pitahaya (Hylocereus undatus) using temporary immersion with liquid culture medium improves micropropagation efficiency compared to the conventional method in semisolid medium. The objective of this work was to evaluate the efficiency of traditional culture and temporary immersion during micropropagation of pitahaya to facilitate the rapid establishment of new commercial plantations of high genetic and phytosanitary quality. Semisolid culture, liquid media in partial immersion and temporary immersion in an Ebb-and-Flow bioreactor were evaluated. Also, in temporary immersion, different immersion frequencies (every 4, 8, 12, and 16 h) and culture densities (5, 10, 15 and 20 explants per bioreactor) were evaluated. For the multiplication stage, new shoots and length were recorded per explant at 45 d of in vitro culture and in the acclimatization stage, the survival percentage was determined at 30 d of greenhouse cultivation. A temporary immersion of 2 min every 4 h and 15 explants per bioreactor was the best culture system, obtaining on average 10.7 shoots per explant with a length of 1.9 cm. No significant differences were observed among treatments during acclimatization, obtaining survival percentages of 98%–100%. This study reports for the first time a protocol for scaling-up techniques in temporary immersion for commercial micropropagation of pitahaya (and for any species of the Cactaceae family) and its establishment in a productive plantation.

临时浸没系统中的放大技术是商业微繁殖的替代方案。火龙果的离体繁殖（火龙果）) 与半固体培养基中的常规方法相比，使用液体培养基临时浸泡提高了微繁殖效率。这项工作的目的是评估火龙果微繁殖期间传统文化和临时浸泡的效率，以促进快速建立具有高遗传和植物检疫质量的新商业种植园。评估了半固体培养、部分浸没和暂时浸没在潮起潮落生物反应器中的液体培养基。此外，在临时浸泡中，评估了不同的浸泡频率（每 4、8、12 和 16 小时）和培养密度（每个生物反应器 5、10、15 和 20 个外植体）。对于繁殖阶段，在体外培养 45 d 和驯化阶段记录每个外植体的新芽和长度，成活率在温室栽培30 d时测定。每 4 小时 2 分钟的临时浸泡和每个生物反应器 15 个外植体是最好的培养系统，每个外植体平均获得 10.7 个芽，长度为 1.9 厘米。在适应过程中，治疗之间没有观察到显着差异，获得的存活百分比为 98%–100%。这项研究首次报告了在临时浸泡中扩大火龙果（以及仙人掌科的任何物种）商业微繁殖及其在生产性种植园中的建立的协议。在适应过程中，治疗之间没有观察到显着差异，获得的存活百分比为 98%–100%。这项研究首次报告了在临时浸泡中扩大火龙果（以及仙人掌科的任何物种）商业微繁殖及其在生产性种植园中的建立的协议。在适应过程中，治疗之间没有观察到显着差异，获得的存活百分比为 98%–100%。这项研究首次报告了在临时浸泡中扩大火龙果（以及仙人掌科的任何物种）商业微繁殖及其在生产性种植园中的建立的协议。