An innovative, ternary nanocomposite composed of overoxidized poly(3,4-ethylenedioxythiophene) (OPEDOT), gold nanoparticles (AuNPs), and electrochemically reduced graphene oxide (ERGO) was prepared on a glassy carbon electrode (GCE) (OPEDOT–AuNPs–ERGO/GCE) through homogeneous chemical reactions and heterogeneous electrochemical methods. The morphology, composition, and structure of this nanocomposite were characterized by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The electrochemical properties of the OPEDOT–AuNPs–ERGO/GCE were investigated by cyclic voltammetry using potassium ferricyanide and hexaammineruthenium(III) chloride redox probe systems. This modified electrode shows excellent electro-catalytic activity for dopamine (DA) and uric acid (UA) under physiological pH conditions, but inhibits the oxidation of ascorbic acid (AA). Linear voltammetric responses were obtained when DA concentrations of approximately 4.0–100 μM and UA concentrations of approximately 20–100 μM were used. The detection limits (S/N=3) for DA and UA were 1.0 and 5.0 μM, respectively, under physiological conditions and in the presence of 1.0 mM of AA. This developed method was applied to the simultaneous detection of DA and UA in human urine, where satisfactory recoveries from 96.7% to 105.0% were observed. This work demonstrates that the developed OPEDOT–AuNPs–ERGO ternary nanocomposite, with its excellent ion-selectivity and electro-catalytic activity, is a promising candidate for the simultaneous detection of DA and UA in the presence of AA in physiological and pathological studies.

在玻璃碳电极 (GCE) (OPEDOT-AuNPs-ERGO) 上制备了一种由过氧化聚 (3,4-乙烯二氧噻吩) (OPEDOT)、金纳米粒子 (AuNPs) 和电化学还原氧化石墨烯 (ERGO) 组成的创新三元纳米复合材料/GCE）通过均相化学反应和异相电化学方法。通过透射电子显微镜、扫描电子显微镜、X射线衍射和X射线光电子能谱对该纳米复合材料的形态、组成和结构进行了表征。OPEDOT-AuNPs-ERGO/GCE 的电化学性质通过循环伏安法使用铁氰化钾和氯化六氨合钌 (III) 氧化还原探针系统进行了研究。这种修饰电极在生理 pH 条件下对多巴胺 (DA) 和尿酸 (UA) 表现出优异的电催化活性，但抑制了抗坏血酸 (AA) 的氧化。当使用约 4.0-100 μM 的 DA 浓度和约 20-100 μM 的 UA 浓度时，获得线性伏安响应。在生理条件下和存在 1.0 mM AA 的情况下，DA 和 UA 的检测限 (S/N=3) 分别为 1.0 和 5.0 μM。这种开发的方法应用于同时检测人尿液中的 DA 和 UA，观察到从 96.7% 到 105.0% 的令人满意的回收率。这项工作表明，开发的 OPEDOT-AuNPs-ERGO 三元纳米复合材料具有优异的离子选择性和电催化活性，