Asparaginases important role in the treatment of leukemia. It is part of chemotherapy in the treatment of leukemia in the last three decades. l-Asparaginase is isolated from Fusarium sp. isolated from soil and purified using ammonium sulfate precipitation and Sephadex G 100. Characterization of the crude enzyme revealed it is a metalloprotease inhibited by EDTA. Hg²⁺, Cd²⁺, and Pb²⁺ also inhibited the enzyme. Mg²⁺, Zn²⁺, and Ca²⁺ activated l-asparaginase. Furthermore, kinetic studies of purified enzyme were carried out. V_max and K_m were 0.031 M and 454 U/mL, respectively. The optimum temperature was 30 °C and the optimum pH was 7. Concerning substrate specificity, gelatin and casein in addition to l-asparagine were tested. The enzyme was found to be nonspecific that could hydrolyze all tested substrates at different rates. The maximum enzyme activity was recorded in the case of l-asparagine, followed by casein and gelatin, respectively. The molecular weight of l-asparaginase was 22.5 kDa. The antileukemic cytotoxicity assay of the enzyme against RAW2674 leukemic cell lines by MTT viability test was estimated. The enzyme exhibited antileukemic activity with IC₅₀ of 50.1 UmL⁻¹. The current work presents additional information regarding the purification and characterization of the enzyme produced by Fusarium sp. and its evaluation as a potential antileukemic chemotherapeutic agent.

天冬酰胺酶在白血病治疗中的重要作用。它是过去三十年治疗白血病的化学疗法的一部分。l-天冬酰胺酶分离自镰刀菌属。从土壤中分离并使用硫酸铵沉淀和 Sephadex G 100 纯化。粗酶的表征表明它是一种受 EDTA 抑制的金属蛋白酶。Hg ²⁺、Cd ²⁺和Pb ²⁺也抑制酶。Mg ²⁺、Zn ²⁺和Ca ²⁺活化的L-天冬酰胺酶。此外，还进行了纯化酶的动力学研究。V _max和K _m分别为 0.031 M 和 454 U/mL。最适温度为 30 °C，最适 pH 值为 7。关于底物特异性，除 L-天冬酰胺外，还测试了明胶和酪蛋白。发现该酶是非特异性的，可以以不同的速率水解所有测试的底物。L-天冬酰胺的酶活性最高，其次是酪蛋白和明胶。L-天冬酰胺酶的分子量为22.5 kDa。通过 MTT 活力试验估计了该酶对 RAW2674 白血病细胞系的抗白血病细胞毒性测定。该酶表现出抗白血病活性，IC ₅₀为 50.1 UmL ^-1. 目前的工作提供了有关镰刀菌产生的酶的纯化和表征的更多信息。及其作为潜在抗白血病化疗剂的评估。