One of the most common dyeing problems of textile industries is uneven and faulty dyeing over the finished quality of fabrics due to different reasons. These problems are usually tackled through chemical degradation in which uneven and faulty dye is removed from the surface of fiber but fabric quality is compromised. Chemical process also reduces the strength of the fabric and durability of textile material by reduction in reactive dye ability. The fabric cannot be reused due to the reduced strength. To overcome above mentioned problem, biological method of stripping in which enzymes produced by different micro-organisms are used. This process has no harmful effect on the fabric and is safe for environment. In this research work reactive blue 21 dye with 0.5, 2 and 4% shade strengths was used to dye cotton fabric. The Ganoderma lucidum fungal strains were mutated by UV mutagen, and five were selected for further processing. These mutant strains were grown at temperature ranges (20 °C to 40 °C); pH(3–5); inoculum size(1–5 mL) and fermentation time (3–15 days) . The required nutrients media to produce the ligninolytic enzymes was added to the flask. The strain which gave the fast decolourization results was selected for further optimization. Optimization was done by observing the variables: incubation time 12 days, pH 4, temperature 30 °C, and inoculum size 3 mL by applying Response Surface Methodology (RSM) in Central Composite Design (CCD). During the process of fabric color stripping, the enzyme assay revealed that the respective mutant UV-60 strain produced active enzymes with their V_max, Mnp (427U/mL), LiP (785U/mL), and Lac (75 U/mL) enzymes decolorized 89% of the dye which is 25% more than the parent strain and also the production of enzyme is Mnp (344U/mL), LiP (693U/mL), and Lac (59 U/mL) enzymes which is lower than mutant strain.

纺织行业最常见的染色问题之一是由于不同原因导致织物成品质量染色不均匀和不合格。这些问题通常通过化学降解来解决，其中不均匀和有缺陷的染料从纤维表面去除，但织物质量会受到影响。化学过程还通过降低活性染料能力来降低织物的强度和纺织材料的耐用性。由于强度降低，织物不能重复使用。为了克服上述问题，使用了由不同微生物产生的酶的生物剥离方法。此工艺对织物无有害影响，对环境安全。在这项研究工作中，使用具有 0.5%、2% 和 4% 色度强度的活性蓝 21 染料对棉织物进行染色。这用紫外线诱变剂对灵芝真菌菌株进行突变，从中选出五个进行进一步加工。这些突变菌株在温度范围（20°C 至 40°C）下生长；pH（3-5）；接种量（1-5 毫升）和发酵时间（3-15 天）。将产生木质素分解酶所需的营养培养基加入烧瓶中。选择提供快速脱色结果的菌株进行进一步优化。通过在中央复合设计 (CCD) 中应用响应面方法 (RSM) 来观察变量：孵育时间 12 天、pH 值 4、温度 30 °C 和接种量 3 mL 来完成优化。在织物脱色过程中，酶分析表明，相应的突变 UV-60 菌株产生了活性酶，其_Vmax、Mnp (427U/mL)、LiP (785U/mL) 和 Lac (75 U/mL) 酶使 89% 的染料脱色，比亲本菌株多 25%，而且酶的产量为 Mnp (344U/mL) mL)、LiP (693U/mL) 和 Lac (59 U/mL) 酶，低于突变株。