As the first line of defense against intestinal bacteria and toxins, intestinal epithelial cells are always exposed to bacteria or lipopolysaccharide (LPS), whereas pathogenic bacteria or LPS can cause intestinal epithelial cell damage. Previous studies have shown that konjac mannan oligosaccharides (KMOS) have a positive effect on maintaining intestinal integrity, and Bacillus subtilis (BS) can promote the barrier effect of the intestine. However, it is still unknown whether KMOS and BS have a synergistic protective effect on the intestines. In this study, we used the LPS-induced Caco-2 cell injury model and mouse intestinal injury model to study the synergistic effects of KMOS and BS. Compared with KMOS or BS alone, co-treatment with KMOS and BS significantly enhanced the activity and antioxidant capacity of Caco-2 cell, protected mouse liver and ileum from LPS-induced oxidative damage, and repaired tight junction and mucus barrier damage by up-regulating the expression of Claudin-1, ZO-1 and MUC-2. Our results demonstrate that the combination of KMOS and BS has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells and aIIeviates LPS-induced acute intestinal injury in mice.

作为肠道细菌和毒素的第一道防线，肠上皮细胞总是暴露在细菌或脂多糖（LPS）中，而致病菌或LPS可引起肠上皮细胞损伤。以往的研究表明，魔芋甘露寡糖（KMOS）对维持肠道完整性有积极作用，枯草芽孢杆菌(BS) 可以促进肠道的屏障作用。然而，KMOS和BS是否对肠道具有协同保护作用尚不清楚。在本研究中，我们使用LPS诱导的Caco-2细胞损伤模型和小鼠肠道损伤模型来研究KMOS和BS的协同作用。与单独使用 KMOS 或 BS 相比，KMOS 和 BS 共同处理显着增强 Caco-2 细胞的活性和抗氧化能力，保护小鼠肝脏和回肠免受 LPS 诱导的氧化损伤，并通过上调修复紧密连接和粘液屏障损伤。调节 Claudin-1、ZO-1 和 MUC-2 的表达。我们的研究结果表明，KMOS 和 BS 的组合对 Caco-2 细胞的炎症和氧化损伤具有协同修复作用，并减轻 LPS 诱导的小鼠急性肠损伤。