Abstract

Dudusa sphingiformis is an important lepidopteran pest widely distributed in tropical and subtropical zones of Asia. In this paper, the complete mitochondrial genome (mitogenome) of D. sphingiformis was determined by next-generation sequencing. The mitogenome was 15,806 bp in length, comprising 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an AT-rich control region (D-loop). The gene arrangement of this mitogenome was identical to that of the previous studies of Notodontidae moths. Almost all the PCGs initiated with typical ATN codons, except for cox1 with CGA. Among them, nine PCGs terminated with TAA or TAG, while other four PCGs (cox1, cox2, nad5, and nad4) with incomplete stop codon T. All the 22 tRNAs had the typical cloverleaf structure, except for trnS1, whose dihydrouridine (DHU) arm forms a simple loop. Phylogenetic analysis based on the concatenated nucleotide sequences of 13 PCGs indicated that D. sphingiformis was more closely related to other species of family Notodontidae, forming a monophyletic group, with well-resolved relationships among five family of Noctuoidea.

摘要

Dudusa sphingiformis是一种重要的鳞翅目害虫，广泛分布于亚洲热带和亚热带地区。在本文中，D. sphingiformis的完整线粒体基因组（mitogenome）是通过二代测序确定的。有丝分裂基因组的长度为 15,806 bp，包括 13 个蛋白质编码基因 (PCG)、22 个 tRNA 基因、两个 rRNA 基因和一个富含 AT 的控制区 (D-loop)。该有丝分裂基因组的基因排列与之前对 Notodontidae 蛾的研究相同。除了带有 CGA 的cox1之外，几乎所有的 PCG 都以典型的 ATN 密码子启动。其中，九周的PCG终止了与TAA或TAG，而其他四个的PCG（COX1，COX2，nad5和nad4) 具有不完整的终止密码子 T。所有 22 种 tRNA 都具有典型的三叶草结构，除了trnS1，其二氢尿苷 (DHU) 臂形成一个简单的环。基于13个PCG的串联核苷酸序列的系统发育分析表明，D. sphingiformis与Notodontidae科其他物种的亲缘关系更密切，形成一个单系群，5个Noctuoidea科之间的关系得到很好的解决。