Objectives

Serotype 2 of dengue virus (DENV-2) is the most prevalent cause of dengue fevers. In this study, the C-prM gene was used for specific detection of DENV-2 by RT-LAMP assay. The RT-LAMP assay was optimized using the Taguchi design of experiments.

Results

The efficiency of the assay in such optimal conditions resulted in 100% sensitivity, 100% specificity, and 100% overall accuracy for detection of 4 copies/μL of the genome of DENV-2. In addition, the detection of 2 copies/μL of the genome of DENV-2 was feasible, although the sensitivity was 50%. Considering the importance of the specific detection of the dengue virus serotypes, the cost-effective RT-LAMP approach can be used for rapid, specific, and sensitive detection of DENV-2.

Conclusion

RT-LAMP, as a cost-effective method, was optimized using Taguchi array approach for specific and rapid detection of DENV-2. Such methods can facilitate the diagnosis procedure in remote regions.

中文翻译：

---

用于敏感和快速检测登革热病毒血清型 2 的逆转录环介导等温扩增 (RT-LAMP) 测定的田口阵列优化

目标

登革热病毒血清型 2 (DENV-2) 是登革热最普遍的原因。在本研究中，C-prM 基因用于通过 RT-LAMP 法特异性检测 DENV-2。使用田口实验设计优化 RT-LAMP 测定。

结果

在这样的最佳条件下测定的效率导致 100% 的灵敏度、 100% 的特异性和 100% 的总体准确度，用于检测 4 拷贝/μL 的 DENV-2 基因组。此外，检测2拷贝/μL的DENV-2基因组是可行的，尽管灵敏度为50%。考虑到特异性检测登革热病毒血清型的重要性，具有成本效益的 RT-LAMP 方法可用于 DENV-2 的快速、特异性和灵敏检测。

结论

RT-LAMP 作为一种具有成本效益的方法，使用田口阵列方法进行了优化，用于特异性和快速检测 DENV-2。这种方法可以促进偏远地区的诊断程序。