Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

中文翻译：

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Hsa_circ_0001947/miR-661/DOK7 轴抑制非小细胞肺癌的发展。

Hsa_circ_0001947 与多种癌症相关，但其在非小细胞肺癌 (NSCLC) 中的作用尚不明确，需要进一步研究。通过数据库预测circ_0001947、miR-661与酪氨酸激酶7（DOK7）下游的靶向关系，并通过双荧光素酶报告基因检测进一步验证，同时通过实时定量聚合酶链检测其在癌组织和细胞中的表达反应 (qRT-PCR)。转染后，分别通过细胞功能实验和qRT-PCR确定miRNA、miR-661和DOK7的细胞生物学行为和表达。Circ_0001947 在 NSCLC 组织和细胞中低表达。Circ_0001947 敲低增强细胞活力和增殖，诱导细胞周期停滞在 S 期，抑制细胞凋亡并明显增强 miR-510、miR-587、miR-661 和 miR-942 水平，而 circ_0001947 过表达则相反。MiR-661是circ_0001947的靶基因，参与circ_0001947对细胞生物学行为的调控。此外，miR-661的靶基因DOK7部分参与了miR-661对细胞活力的调控。Hsa_circ_0001947 作为 miR-661 的海绵通过提高 DOK7 的表达来抑制 NSCLC 的发展。