Purity and integrity are two important criteria for any RNA extraction process to qualify the RNA for meaningful gene expression analysis. This study compares four commercially available RNA extraction kits using silica membrane and magnetic bead separation methods. The performance was evaluated in terms of both quantity (total RNA amount in μg/μl) and purity (260/280 ratio). The concentration and purity of each kit was significantly different from those of the others (p < 0.001). Although quantity obtained from Mag MAX is comparatively lower than QIAGEN, the quality is comparable as evident from real-time PCR performance. This study suggests that there are practical differences between these RNA extraction kits that should be taken into account while isolating RNA required for gene expression analysis.

中文翻译：

---

通过使用两种不同的化学物质产生高质量的病毒 RNA：比较性能研究。

纯度和完整性是任何 RNA 提取过程的两个重要标准，用于对 RNA 进行有意义的基因表达分析。本研究比较了四种使用硅胶膜和磁珠分离方法的市售 RNA 提取试剂盒。根据数量（以 μg/μl 为单位的总 RNA 量）和纯度（260/280 比率）评估性能。每个试剂盒的浓度和纯度与其他试剂盒的浓度和纯度显着不同（p < 0.001）。尽管从 Mag MAX 获得的数量相对低于 QIAGEN，但从实时 PCR 性能来看，质量相当。这项研究表明，在分离基因表达分析所需的 RNA 时，应考虑这些 RNA 提取试剂盒之间的实际差异。