Even with recent advances in lineage tracing and multiomics analyses, it is difficult to specifically identify and target heterogeneous fibroblast populations in an organ-specific manner. This study combined robust multi-organ in vitro, in vivo, and high-throughput gene expression analyses of mouse fibroblast populations derived from various organs to define organ-specific transcriptional signatures. The analyses revealed that fibroblasts derived from different organs retain transcriptional embryonic gene signatures as organ-of-origin identifiers in adulthood. Moreover, organ-specific transcriptome identities of fibroblast populations were maintained in fibroblast co-cultures in vitro and ectopic transplants in vivo. These studies advance our current understanding of the heterogeneity of fibroblast populations and suggest opportunities for their exploitation and targetability in an organ-specific manner.

中文翻译：

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预印本亮点：成体成纤维细胞保留器官特异性转录组学特性。

即使在谱系追踪和多组学分析方面取得了最新进展，也很难以器官特异性方式特异性识别和靶向异质成纤维细胞群。该研究结合了来自各种器官的小鼠成纤维细胞群的强大的体外、体内和高通量基因表达分析，以确定器官特异性转录特征。分析表明，来自不同器官的成纤维细胞保留了转录胚胎基因特征，作为成年后的器官来源标识符。此外，成纤维细胞群的器官特异性转录组特性在体外成纤维细胞共培养和体内异位移植中得以保持。