Whole-genome sequence analysis of noroviruses is routinely performed by employing a metagenomic approach. While this methodology has several advantages, such as allowing for the examination of co-infection, it has some limitations, such as the requirement of high viral load to achieve full-length or near full-length genomic sequences. In this study, we used a pre-amplification step to obtain full-length genomic amplicons from 39 Canadian GII isolates, followed by deep sequencing on Illumina and Oxford Nanopore platforms. This approach significantly reduced the required viral titre to obtain full-genome coverage. Herein, we compared the coverage and sequences obtained by both platforms and provided an in-depth genomic analysis of the obtained sequences, including the presence of single-nucleotide variants and recombination events.

中文翻译：

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使用组合的 Illumina-Nanopore 数据对人类诺如病毒进行基因组分析

诺如病毒的全基因组序列分析通常采用宏基因组方法进行。虽然这种方法有几个优点，例如允许检查共感染，但它也有一些限制，例如需要高病毒载量才能实现全长或接近全长的基因组序列。在这项研究中，我们使用预扩增步骤从 39 个加拿大 GII 分离株中获得全长基因组扩增子，然后在 Illumina 和 Oxford Nanopore 平台上进行深度测序。这种方法显着降低了获得全基因组覆盖所需的病毒滴度。在这里，我们比较了两个平台获得的覆盖率和序列，并对获得的序列进行了深入的基因组分析，包括单核苷酸变体和重组事件的存在。