The increased interest of consumers in probiotic foods requires a deeper knowledge on the possible interactions with drugs, because their pharmacological properties could be modified. In this context, these studies are relevant for drugs such as acenocoumarol, whose dosage must be controlled due to, among other factors, food-drug interactions. Acenocoumarol is an oral anticoagulant with a narrow therapeutic range. The aim of the present research is to evaluate, in vitro, the effect of bifidobacteria on acenocoumarol. The drug was incubated with Bifidobacterium bifidum CIDCA 5310 or Bifidobacterium adolescentis CIDCA 5317 in MRS broth at 37°C for 24 h in anaerobic conditions. The effect of incubation with sterilized spent culture supernatants (SSCS) was also evaluated. Analysis by RP-HPLC showed that both bifidobacterial strains reduced the area of the acenocoumarol peak and two new peaks were evidenced. In addition, a decrease in the intensity of the bands at 1650, 1390 and 1110/cm was observed in the FTIR spectroscopic determinations. Moreover, a new band appeared at 1720/cm. No effect on the drug was observed when incubation was performed with SSCS. The present study showed a significant change in the concentration of the anticoagulant after incubation with bifidobacteria and results are compatible with biomodification of the drug due to enzymatic activity of bifidobacteria.

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双歧杆菌对醋硝香豆素的生物修饰

消费者对益生菌食品越来越感兴趣，需要对可能与药物的相互作用有更深入的了解，因为它们的药理特性可以改变。在这种情况下，这些研究与醋硝香豆素等药物相关，由于食品-药物相互作用等因素，必须控制其剂量。Acenocoumarol 是一种口服抗凝剂，治疗范围较窄。本研究的目的是在体外评估双歧杆菌对醋硝香豆素的影响。将药物与双歧双歧杆菌CIDCA 5310 或青春双歧杆菌一起孵育CIDCA 5317 在 MRS 肉汤中在 37°C 厌氧条件下培养 24 小时。还评估了用灭菌的用过的培养物上清液 (SSCS) 孵育的效果。RP-HPLC 分析表明，两种双歧杆菌菌株都减少了醋硝香豆素峰的面积，并证实了两个新峰。此外，在 FTIR 光谱测定中观察到 1650、1390 和 1110/cm 处的谱带强度降低。此外，在 1720/cm 处出现了一个新带。当与 SSCS 一起进行孵育时，未观察到对药物的影响。本研究表明，在与双歧杆菌孵育后，抗凝剂的浓度发生了显着变化，并且由于双歧杆菌的酶活性，结果与药物的生物修饰相一致。