Background: Establishing R, S-enantiomer (S-KT and R-KT) chiral resolution and determination methods for KT are of great significance.

Objective: This study aimed to establish a high-performance liquid chromatography (HPLC) method for the resolution and determination of ketorolac tromethamine (KT) enantiomers.

Methods: A CHIRALPAK AGP column (0.4 × 10 cm, 5 μm) was used with 10 mmol/L ammonium acetate (pH 5.5) and isopropanol (97:3) as the mobile phase. The detection wavelength was 324 nm, the flow rate was 1.0 mL/min, the column temperature was 25°C, and the injection volume was 5 μL.

Results: The resolution between S-KT and R-KT was 2.8. S-KT and R-KT demonstrated a good linear relationship in the range of 3-60 μg/mL (r > 0.999). The average recoveries of S-KT and RKT were 99.2% and 99.8%, with relative standard deviations of 2.0% and 2.4%, respectively.

Conclusion: The established method can be used for the resolution and determination of S-KT and R-KT.

背景：建立R、S-对映体（S-KT和R-KT）手性拆分和KT的测定方法具有重要意义。

目的：本研究旨在建立一种用于拆分和测定酮咯酸氨丁三醇（KT）对映体的高效液相色谱（HPLC）方法。

方法：CHIRALPAK AGP 色谱柱（0.4 × 10 cm，5 μm），流动相为 10 mmol/L 醋酸铵（pH 5.5）和异丙醇（97:3）。检测波长为324 nm，流速为1.0 mL/min，柱温为25°C，进样量为5 μL。

结果：S-KT和R-KT的分辨率为2.8。S-KT 和 R-KT 在 3-60 μg/mL 范围内表现出良好的线性关系 (r > 0.999)。S-KT 和 RKT 的平均回收率分别为 99.2% 和 99.8%，相对标准偏差分别为 2.0% 和 2.4%。

结论：所建立的方法可用于S-KT和R-KT的分离和测定。