Growing evidence implicates an association between psychosocial stress and oxidative stress (OxSt) although there are not yet reliable biomarkers to study this association. We used a Trier Social Stress Test (TSST) and compared the response of a healthy control group (HC; N=10) against the response of a schizophrenia group (SCZ; N=10) that is expected to have higher levels of OxSt. Because our previous study showed inconsistent changes in conventional molecular markers for stress responses in the neuroendocrine and immune systems, we analyzed the same serum samples using a separate reducing capacity assay that provides a more global measurement of OxSt. This assay uses the moderately strong oxidizing agent iridium (Ir) to probe a sample's reducing capacity. Specifically, we characterized OxSt by this Ir-reducing capacity assay (Ir-RCA) using two measurement modalities (optical and electrochemical) and we tuned this assay by imposing an input voltage sequence that generates multiple output metrics for data-driven analysis. We defined five OxSt metrics (one optical and four electrochemical metrics) and showed: (i) internal consistency among each metric in the measurements of all 40 samples (baseline and post TSST for N=20); (ii) all five metrics were consistent with expectations of higher levels of OxSt for the SCZ group (three individual metrics showed statistically significant differences); and (iii) all five metrics showed higher levels of OxSt Post-TSST (one metric showed statistically significant difference). Using multivariant analysis, we showed that combinations of OxSt metrics could discern statistically significant increases in OxSt for both the SCZ and HC groups 90 min after the imposed acute psychosocial stress.

越来越多的证据表明社会心理压力和氧化应激 (OxSt) 之间存在关联，尽管目前还没有可靠的生物标志物来研究这种关联。我们使用了特里尔社会压力测试 (TSST)，并将健康对照组（HC；N=10）的反应与预期具有较高 OxSt 水平的精神分裂症组（SCZ；N=10）的反应进行了比较。由于我们之前的研究显示神经内分泌和免疫系统中应激反应的常规分子标记发生了不一致的变化，我们使用单独的还原能力测定分析了相同的血清样本，该测定提供了更全面的 OxSt 测量。该测定使用中等强度的氧化剂铱 (Ir) 来探测样品的还原能力。具体来说，我们使用两种测量方式（光学和电化学）通过这种 Ir 还原能力测定 (Ir-RCA) 对 OxSt 进行了表征，我们通过施加输入电压序列来调整该测定，该序列可生成用于数据驱动分析的多个输出指标。我们定义了五个 OxSt 指标（一个光学指标和四个电化学指标）并表明：（i）在所有 40 个样本的测量中每个指标之间的内部一致性（N=20 的基线和 TSST 后）；(ii) 所有五个指标都与 SCZ 组更高水平的 OxSt 的预期一致（三个单独的指标显示出统计学上的显着差异）；(iii) 所有五个指标都显示出更高水平的 OxSt Post-TSST（一个指标显示出统计学上的显着差异）。使用多变量分析，