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Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients
Signal Transduction and Targeted Therapy ( IF 40.8 ) Pub Date : 2021-09-16 , DOI: 10.1038/s41392-021-00753-7
Xiang-Na Zhao 1 , Yue You 2, 3 , Xiao-Ming Cui 4 , Hui-Xia Gao 5 , Guo-Lin Wang 4 , Sheng-Bo Zhang 2, 3 , Lin Yao 4 , Li-Jun Duan 4 , Ka-Li Zhu 4 , Yu-Ling Wang 5 , Li Li 5 , Jian-Hua Lu 5 , Hai-Bin Wang 5 , Jing-Fang Fan 5 , Huan-Wei Zheng 5 , Er-Hei Dai 5 , Lu-Yi Tian 2, 3 , Mai-Juan Ma 4
Affiliation  

While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56briCD16 natural killer (NK) cells and upregulation of interferon-gamma in effector CD4+ and CD8+ T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4+ T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.



中文翻译:

单细胞免疫分析揭示了无症状 COVID-19 患者的不同免疫反应

虽然一些被严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 感染的个体呈现轻到重度的疾病,但许多 SARS-CoV-2 感染的个体是无症状的。我们试图确定无症状和中度患者之间免疫反应的区别。我们对来自健康对照的无症状、中度和重度患者的 37 个纵向收集的外周血单核细胞样本进行了单细胞转录组和 T 细胞/B 细胞受体 (TCR/BCR) 测序。无症状患者表现出 CD56 bri CD16 -自然杀伤 (NK) 细胞增加和效应子 CD4 +和 CD8 +中干扰素-γ 的上调T细胞和NK细胞。他们表现出更强大的 TCR 克隆扩增,尤其是在效应 CD4 + T 细胞中,但与中度患者相比缺乏强大的 BCR 克隆扩增。此外,无症状患者的干扰素刺激基因 (ISG) 表达一般较低,但患者间变异性较大,而中度患者在多个细胞群中显示出 ISG 表达的不同幅度和时间动态,但低于严重疾病患者。我们的数据提供了无症状感染中 SARS-CoV-2 不同免疫特征的证据。

更新日期:2021-09-16
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