Airway surface liquid (ASL) is one of the key factors affecting the respiratory system's physiological function. Abnormal ASL secretion can increase the incidence of various respiratory diseases. Lipopolysaccharide (LPS) stimulation can damage the airway epithelial barrier, affect the concentration of ASL contents, and down-regulate ion channel expression, which in turn causes abnormal ASL secretion. Naringenin, which exists in many Citrus foods, has the ability to promote airway surface liquid secretion. This work is designed to investigate the regulatory mechanism of naringenin on LPS-induced abnormal ASL secretion. The effects of naringenin and LPS on the viability of Calu-3 cells were measured by CellTiter 96^® AQueous One Solution Cell Proliferation Assay (MTS). ASL secretion volume was measured by a micropipette on air–liquid interface cultured cells. The concentration of Cl⁻, Na⁺, lysozyme, and total protein in ASL were respectively measured by assay kits. The mRNA expressions were determined by quantitative real-time polymerase chain reaction, and proteins were measured by enzyme-linked immunosorbent assay. The results indicated that LPS could affect ASL secretion and regulate cystic fibrosis transmembrane conductance regulator (CFTR), aquaporin 1 (AQP1) and aquaporin 5 (AQP5) expression. Naringenin had the ability to regulate the ASL secretion by increasing secretion volume, and Cl⁻ and Na⁺ concentrations, reducing lysozyme and total protein content, and regulating CFTR, AQP1, and AQP5 expression. This study indicated that naringenin had regulating effects to attenuate LPS-induced abnormal ASL secretion.

气道表面液体（ASL）是影响呼吸系统生理功能的关键因素之一。ASL分泌异常会增加各种呼吸系统疾病的发病率。脂多糖（LPS）刺激可破坏气道上皮屏障，影响 ASL 内容物浓度，下调离子通道表达，进而导致 ASL 分泌异常。柚皮素存在于许多柑橘类食物中，具有促进气道表面液体分泌的能力。这项工作旨在研究柚皮素对 LPS 诱导的异常 ASL 分泌的调节机制。CellTiter 96 ^®测量柚皮素和LPS对Calu-3细胞活力的影响一种水溶液细胞增殖试验（MTS）。ASL 分泌量通过微量移液管在气液界面培养细胞上测量。用试剂盒分别测定ASL中Cl ^-、Na ⁺、溶菌酶和总蛋白的浓度。mRNA 表达通过定量实时聚合酶链反应测定，蛋白质通过酶联免疫吸附法测定。结果表明LPS可以影响ASL的分泌并调节囊性纤维化跨膜电导调节剂（CFTR）、水通道蛋白1（AQP1）和水通道蛋白5（AQP5）的表达。柚皮素具有通过增加分泌量来调节 ASL 分泌的能力，Cl ^-和 Na ⁺浓度，降低溶菌酶和总蛋白含量，并调节 CFTR、AQP1 和 AQP5 的表达。该研究表明柚皮素具有减轻 LPS 诱导的异常 ASL 分泌的调节作用。