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Facile fabrication of soy protein isolate-functionalized nanofibers with enhanced biocompatibility and hemostatic effect on full-thickness skin injury
Nanoscale ( IF 5.8 ) Pub Date : 2021-09-01 , DOI: 10.1039/d1nr03430h
Meifang Ke 1 , Zijian Wang 2, 3 , Qi Dong 1 , Feixiang Chen 1 , Liu He 1 , Céline Huselstein 4 , Xinghuan Wang 2, 3 , Yun Chen 1
Affiliation  

Extensive full-thickness skin defect lacks self-healing ability. Tissue engineering wound dressing is considered as the most promising approach to promote wound healing. In this study, a series of biocompatible and hemostatic nanofiber dressings were fabricated. Soy protein isolate (SPI) and poly(L-lactic acid) (PLLA) solutions were mixed in certain proportions for high-voltage electrospinning. The obtained products were coded as SPNF-n (n = 100, 80, 60 and 40, corresponding to the weight percentage of PLLA solution). We found that SPNF-n (n = 100, 80, 60 and 40) could facilitate the adhesion and spread of L929 cells. In particular, SPNF-80 was capable of promoting fibroblast proliferation and diminishing inflammation. Compared with the neat PLLA film (SPNF-100), the biosafety and hemostatic effect of SPNF-80 got significantly improved. The hemostatic effect of SPNF-80 was comparable with that of a commercial gelatin sponge. In vivo wound healing assay demonstrated that SPNF-80 could accelerate the wound healing process by enhancing vascularization, re-epithelization and collagen formation. In conclusion, our results reveal that SPNF-n has good biocompatibility and hemostatic effect, and exhibits great application potential in wound healing.

中文翻译:

轻松制备具有增强生物相容性和止血作用的大豆分离蛋白功能化纳米纤维对全层皮肤损伤

大面积全层皮肤缺损缺乏自愈能力。组织工程伤口敷料被认为是促进伤口愈合的最有前途的方法。在这项研究中,制造了一系列生物相容性和止血纳米纤维敷料。大豆分离蛋白(SPI)和聚(L-乳酸)(PLLA)溶液按一定比例混合用于高压静电纺丝。所得产物编码为 SPNF- nn = 100、80、60和 40,对应于 PLLA 溶液的重量百分比)。我们发现 SPNF- n ( n= 100、80、60 和 40) 可以促进 L929 细胞的粘附和扩散。特别是,SPNF-80 能够促进成纤维细胞增殖和减少炎症。与纯PLLA膜(SPNF-100)相比,SPNF-80的生物安全性和止血效果得到显着提高。SPNF-80 的止血效果与商业明胶海绵的止血效果相当。体内伤口愈合试验表明,SPNF-80 可以通过增强血管化、再上皮化和胶原形成来加速伤口愈合过程。总之,我们的研究结果表明,SPNF- n具有良好的生物相容性和止血作用,在伤口愈合中具有很大的应用潜力。
更新日期:2021-09-16
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