Design and development of plastic antibodies against SARS-CoV-2 RBD based on molecularly imprinted polymers that inhibit in vitro virus infection,Nanoscale

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Design and development of plastic antibodies against SARS-CoV-2 RBD based on molecularly imprinted polymers that inhibit in vitro virus infection
Nanoscale ( IF 5.8 ) Pub Date : 2021-08-13 , DOI: 10.1039/d1nr03727g
Ortensia Ilaria Parisi _{1,

2} , Marco Dattilo ₁ , Francesco Patitucci ₁ , Rocco Malivindi _{1,

2} , Serena Delbue ₃ , Pasquale Ferrante ₃ , Silvia Parapini ₄ , Roberta Galeazzi ₅ , Mariangela Cavarelli ₆ , Francesco Cilurzo ₇ , Silvia Franzè ₇ , Ida Perrotta ₈ , Vincenzo Pezzi _{1,

2} , Francesca Selmin ₇ , Mariarosa Ruffo _{1,

2} , Francesco Puoci _{1,

2}

Affiliation

The present research study reports the development of plastic antibodies based on Molecularly Imprinted Polymers (MIPs) capable of selectively binding a portion of the novel coronavirus SARS-CoV-2 spike protein. Indeed, molecular imprinting represents a very promising and attractive technology for the synthesis of MIPs characterized by specific recognition abilities for a target molecule. Given these characteristics, MIPs can be considered tailor-made synthetic antibodies obtained by a templating process. After in silico analysis, imprinted nanoparticles were synthesized by inverse microemulsion polymerization and their ability to prevent the interaction between ACE2 and the receptor-binding domain of SARS-CoV-2 was investigated. Of relevance, the developed synthetic antibodies are capable of significantly inhibiting virus replication in Vero cell culture, suggesting their potential application in the treatment, prevention and diagnosis of SARS-CoV-2 infection.

中文翻译：

基于抑制体外病毒感染的分子印迹聚合物设计和开发针对 SARS-CoV-2 RBD 的塑料抗体

本研究报告了基于分子印迹聚合物 (MIP) 的塑料抗体的开发，该抗体能够选择性地结合部分新型冠状病毒 SARS-CoV-2 刺突蛋白。事实上，分子印迹代表了一种非常有前途和有吸引力的合成 MIP 的技术，其特征是对目标分子的特定识别能力。鉴于这些特征，MIP 可以被视为通过模板过程获得的量身定制的合成抗体。之后，在硅片分析，通过反相微乳液聚合合成了印迹纳米颗粒，并研究了它们阻止 ACE2 与 SARS-CoV-2 受体结合域之间相互作用的能力。相关的是，开发的合成抗体能够显着抑制 Vero 细胞培养物中的病毒复制，表明它们在 SARS-CoV-2 感染的治疗、预防和诊断中具有潜在的应用价值。

更新日期：2021-09-16

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