Background: Gastric cancer (GC) is among the most prevalent cancers globally. As such, there is a need to explore the mechanism underlying its pathogenesis and identify potential biomarkers for its prognosis.
Methods: ONCOMINE was used to screen differentially expressed genes between GC and normal gastric mucosa. GEPIA was used to analyze the expression and correlation of candidate genes in tumor node metastasis (TNM) stage. STRING was used to construct protein interaction network. Kaplan–Meier plotter was used to analyze survival. TIMER was used to evaluate the association between candidate genes and immune cell infiltration.
Results: From the ONCOMINE database, we found COL1A1, COL1A2, COL6A3, and SULF1 genes were significantly upregulated in stomach adenocarcinomas. There was a considerable correlation between the expression of COL1A1 (p = 0.029), COL1A2 (p = 0.004), COL6A3 (p = 0.002), SULF1 (p = 0.001), and the TNM stage. COL1A1 was positively correlated with ERBB2 (R = − 0.037, p = 0.46), while the other three genes were negatively correlated with ERBB2 (p > 0.05). The Kaplan–Meier plotter showed that low transcriptional levels of COL1A1 (p = 0.0020), COL1A2 (p = 0.0015), COL6A3 (p = 0.0015), and SULF1 (p = 0.0016) in gastric cancer patients were remarkably related to longer overall survival. In addition, there was a close relationship between chemokine expression and infiltration of the six immune cell types: B cells, macrophages, CD4+ T cells, CD8+ T cells, dendritic cells, and neutrophils, implying that the genes acted as indicators of both prognosis and immune status.
Conclusion: Our findings implicate COL1A1, COL1A2, COL6A3, and SULF1 as candidate biomarkers for the prognosis of gastric cancer.



中文翻译：

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COL1A1、COL1A2、COL6A3 和 SULF1 作为胃癌预后生物标志物的差异表达

背景：胃癌（GC）是全球最常见的癌症之一。因此，有必要探索其发病机制并确定其预后的潜在生物标志物。
方法：采用ONCOMINE筛选GC与正常胃黏膜的差异表达基因。GEPIA用于分析候选基因在肿瘤淋巴结转移（TNM）阶段的表达和相关性。STRING用于构建蛋白质相互作用网络。Kaplan-Meier 绘图仪用于分析存活率。TIMER用于评估候选基因与免疫细胞浸润之间的关联。
结果：从 ONCOMINE 数据库中，我们发现 COL1A1、COL1A2、COL6A3 和 SULF1 基因在胃腺癌中显着上调。COL1A1 (p = 0.029)、COL1A2 (p = 0.004)、COL6A3 (p = 0.002)、SULF1 (p = 0.001) 的表达与 TNM 分期之间存在相当大的相关性。COL1A1 与 ERBB2 呈正相关（R = - 0.037，p = 0.46），而其他三个基因与 ERBB2 呈负相关（p > 0.05）。Kaplan-Meier 绘图仪显示，胃癌患者中 COL1A1 (p = 0.0020)、COL1A2 (p = 0.0015)、COL6A3 (p = 0.0015) 和 SULF1 (p = 0.0016) 的低转录水平与较长的总生存期显着相关. 此外，趋化因子表达与六种免疫细胞类型的浸润密切相关：B 细胞、巨噬细胞、CD4+ T 细胞、CD8+ T 细胞、
结论：我们的研究结果表明 COL1A1、COL1A2、COL6A3 和 SULF1 作为胃癌预后的候选生物标志物。