Development of a qPCR-based method for counting overwintering spruce budworm (Choristoneura fumiferana) larvae collected during fall surveys and for assessing their natural enemy load: a proof-of-concept study,Pest Management Science

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Development of a qPCR-based method for counting overwintering spruce budworm (Choristoneura fumiferana) larvae collected during fall surveys and for assessing their natural enemy load: a proof-of-concept study
Pest Management Science ( IF 3.8 ) Pub Date : 2021-09-16 , DOI: 10.1002/ps.6645
Abdelmadjid Djoumad ₁ , Philippe Tanguay ₁ , Jacques Régnière ₁ , Guylaine Trudel ₂ , Andrew Morrison ₃ , Cédric Fournier ₂ , Drew Carleton ₃ , Audrey Nisole ₁ , Don Stewart ₁ , Michel Cusson _{1,

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Affiliation

In eastern Canada, surveys of overwintering 2^nd instar spruce budworm (Choristoneura fumiferana) larvae (‘L2s’) are carried out each fall to guide insecticide application decisions in the following spring. These surveys involve the collection of fir and spruce branches in selected stands, followed by the mechanical/chemical removal of larvae. The latter then are counted manually on filter papers, using a stereomicroscope. Considering the significant effort and difficulties which this manual counting entails, we developed a quantitative (q)PCR-based ‘molecular counting’ approach designed to make this step less tedious.

中文翻译：

开发基于 qPCR 的方法，用于计算秋季调查期间收集的越冬云杉芽虫 (Choristoneura fumiferana) 幼虫并评估其天敌负荷：概念验证研究

在加拿大东部，每年秋季都会对越冬的二龄云杉芽虫 ( Choristoneura fumiferana ⁾幼虫 ('L2s') 进行调查，以指导次年春季的杀虫剂施用决策。这些调查涉及在选定的林分中收集冷杉和云杉树枝，然后机械/化学去除幼虫。然后使用立体显微镜在滤纸上手动计数后者。考虑到这种手动计数需要付出巨大的努力和困难，我们开发了一种基于定量 (q)PCR 的“分子计数”方法，旨在减少这一步骤的繁琐。

更新日期：2021-09-16

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