Nanohybrids of two-dimensional (2D) layered materials have shown fascinating prospects towards the fabrication of highly efficient fluorescent immunosensor. In this context, a nanohybrid of ultrathin Ti₃C₂-MXene nanosheets and silver nanoparticles (Ag@Ti₃C₂-MXene) has been reported as a dual-energy acceptor for ultrahigh fluorescence quenching of protein-functionalized graphene quantum dots (anti-NSE/amino-GQDs). The Ti₃C₂-MXene nanosheets are decorated with silver nanoparticles (AgNPs) to obsolete the agglomeration and restacking through a one-pot direct reduction method wherein the 2D Ti₃C₂-MXene nanosheets acted both as a reducing agent and support matrix for AgNPs. The as-prepared nanohybrid is characterized by various techniques to analyze the optical, structural, compositional, and morphological parameters. The quenching efficiency and energy transfer capability between the anti-NSE/amino-GQDs (donor) and Ag@Ti₃C₂-MXene (acceptor) have been explored through steady state and time-resolved spectroscopic studies. Interestingly, the Ag@Ti₃C₂-MXene nanohybrid exhibits better quenching and energy transfer efficiencies in contrast to bare Ti₃C₂-MXene, AgNPs and previously reported AuNPs. Based on optimized donor-acceptor pair, a fluorescent turn-on biosensing system is constructed that revealed improved biosensing characteristics compared to Ti₃C₂-MXene, graphene and AuNPs for the detection of neuron-specific enolase (NSE), including higher sensitivity (∼771 mL ng⁻¹), broader linear detection range (0.0001–1500 ng mL⁻¹), better LOD (0.05 pg mL⁻¹), and faster response time (12 min). Besides, remarkable biosensing capability has been observed in serum samples, with fluorescence recovery of ∼98%.

二维 (2D) 层状材料的纳米杂化物在制造高效荧光免疫传感器方面显示出迷人的前景。在此背景下，超薄 Ti ₃ C ₂ -MXene 纳米片和银纳米粒子（Ag@Ti ₃ C ₂ -MXene）的纳米混合物已被报道为双能受体，用于蛋白质功能化石墨烯量子点（抗-NSE/氨基-GQDs）。Ti ₃ C ₂ -MXene 纳米片用银纳米粒子 (AgNPs) 装饰，以通过一锅法直接还原方法消除团聚和重新堆积，其中 2D Ti ₃ C ₂-MXene 纳米片既充当还原剂又充当 AgNP 的支撑基质。所制备的纳米杂化物的特点是通过各种技术来分析光学、结构、成分和形态参数。已经通过稳态和时间分辨光谱研究探索了抗 NSE/氨基 GQD（供体）和 Ag@Ti ₃ C ₂ -MXene（受体）之间的猝灭效率和能量转移能力。有趣的是，与裸 Ti ₃ C ₂相比，Ag@Ti ₃ C ₂ -MXene 纳米杂化物表现出更好的淬火和能量转移效率-MXene、AgNPs 和之前报道的 AuNPs。基于优化的供体-受体对，构建了一种荧光开启生物传感系统，与用于检测神经元特异性烯醇化酶 (NSE) 的Ti ₃ C ₂ -MXene、石墨烯和 AuNP相比，该系统显示出更好的生物传感特性，包括更高的灵敏度。 ∼771 mL ng ^-1 )、更宽的线性检测范围 (0.0001–1500 ng mL ^-1 )、更好的 LOD (0.05 pg mL ^-1 ) 和更快的响应时间 (12 分钟)。此外，在血清样品中观察到了显着的生物传感能力，荧光回收率约为 98%。