Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized. The insulin (INS) was immobilized on the surface of amine-modified silica particles employing covalent attachment by EDC/NHS chemistry and via electrostatic interaction. The resulting formulations were examined for the morphology (SEM), chemical composition (FTIR, XPS) as well as protein content. To facilitate the injectability and support the bone regeneration, developed particles were dispersed in biopolymeric sol composed of collagen, chitosan and lysine-modified hyaluronic acid and crosslinked with genipin. By means of rheological study, the sol-gel in situ transition of obtained systems was verified. It was found in vitro study that MG-63 cells cultured on the developed composites exhibit significantly higher alkaline phosphatase (ALP) activity, compared to the pristine hydrogel. Furthermore, the biomineralization ability in the simulated body fluid (SBF) model was also demonstrated. Our findings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects, especially as a less-cost effective support/alternative for BMP-2 systems.

合成了基于用胰岛素功能化二氧化硅颗粒增强的生物聚合物水凝胶的新型生物活性可注射复合材料。胰岛素 (INS) 通过 EDC/NHS 化学和静电相互作用采用共价连接固定在胺改性二氧化硅颗粒的表面。检查所得制剂的形态 (SEM)、化学成分 (FTIR、XPS) 以及蛋白质含量。为了促进可注射性和支持骨再生，将开发的颗粒分散在由胶原蛋白、壳聚糖和赖氨酸修饰的透明质酸组成的生物聚合物溶胶中，并与京尼平交联。通过流变学研究，验证了所得体系的溶胶-凝胶原位转变。它在体外被发现研究表明，与原始水凝胶相比，在开发的复合材料上培养的 MG-63 细胞表现出显着更高的碱性磷酸酶 (ALP) 活性。此外，还证明了模拟体液（SBF）模型中的生物矿化能力。我们的研究结果表明，本文提出的新型水凝胶复合材料可能是骨缺损再生的有前途的配方，尤其是作为 BMP-2 系统的低成本有效支持/替代品。