In the current study, the silver nanoparticles are green synthesized using reducing and stabilizing potential of aqueous extract of Linseed. The Response Surface Methodology (RSM) along with central composite design (CCD) was used to evaluate the influence of various independent variables like the concentration of silver nitrate (AgNO₃), Linseed Hydrogel (LSH) suspensiot not used to find out the relative influencn percentage, sonication of LSH suspension, and exposure of reaction mixture to the sunlight on the response variable. RSM interpretation revealed that the results of all experiments were best fitted into a quadratic polynomial model with R² value 0.9978 with SD 0.0018 for all responses. The optimized preparation conditions for LSH-Ag nanoparticles were concentration of AgNO₃ (8.68 mM), LSH suspension percentage (0.73%), sonication time (5.29 min), and exposure to sunlight (69.52 min). The resultant LSH-Ag NPs were characterized using UV-Vis spectroscopy, ATR-FTIR, BT-90 nano size analyzer, PXRD, and SEM. These results illustrated that LSH-Ag NPs had a spherical shape, average size 34.47 nm, maximum absorbance at 423 nm, polydispersity index 0.063. Afterward, the anti-bacterial and anti-biofilm activity of LSH-Ag NPs was investigated against E. coli and B. subtilis whereas scavenging activity of 84.2% was shown by NPs with DPPH assay. The result of biological activities proposed synthesized NPs as apposite material having capability to be used as biological agent. The biological potential can be depicted by its phytochemical profile having total phenolic and flavonoid contents as 84.9 mg GA/g and 45.9 mg RU/g respectively. The thin film of synthesized NPs possesses tiny cavities that suggested it to use in drug delivery system.

在目前的研究中，银纳米粒子是使用亚麻籽水提取物的还原和稳定电位合成的绿色纳米粒子。响应面法 (RSM) 和中心复合设计 (CCD) 用于评估各种自变量的影响，如硝酸银 (AgNO ₃ )的浓度、未使用的亚麻籽水凝胶 (LSH) 悬浮液，以找出相对影响。百分比、LSH 悬浮液的超声处理以及反应混合物在响应变量上的阳光照射。RSM 解释表明，所有实验的结果最适合所有响应的R ²值为 0.9978 且 SD 为 0.0018的二次多项式模型。LSH-Ag纳米粒子的优化制备条件为AgNO浓度₃ (8.68 mM)、LSH 悬浮百分比 (0.73%)、超声处理时间 (5.29 分钟) 和暴露在阳光下 (69.52 分钟)。使用 UV-Vis 光谱、ATR-FTIR、BT-90 纳米尺寸分析仪、PXRD 和 SEM 对所得 LSH-Ag NP 进行表征。这些结果表明 LSH-Ag NPs 呈球形，平均尺寸为 34.47 nm，最大吸光度为 423 nm，多分散指数为 0.063。然后，研究了 LSH-Ag NPs 对大肠杆菌和枯草芽孢杆菌的抗菌和抗生物膜活性而使用 DPPH 测定的 NPs 显示出 84.2% 的清除活性。生物活性的结果表明合成的 NPs 作为合适的材料具有用作生物制剂的能力。生物潜力可以通过其总酚类和类黄酮含量分别为 84.9 mg GA/g 和 45.9 mg RU/g 的植物化学特征来描述。合成的 NPs 薄膜具有微小的空腔，表明它可以用于药物输送系统。