This study was to evaluate the expression of miR-129–5p in non-alcoholic fatty liver (NAFLD) patients and its clinical value and explore its regulatory effect on insulin resistance (IR). A total of 117 NAFLD patients and 110 healthy controls were included. The levels of miR-129-5p were detected by qRT-PCR. To assess the diagnostic value of miR-129-5p for NAFLD, the receiver operating characteristic curve (ROC) was established. C57Bl/6 mice were supplied with high-fat diet to establish NAFLD model. Intraperitoneal insulin tolerance test (IPITT) was carried out to evaluate the effect of miR-129-5p on IR in NAFLD animal model. miR-129-5p was highly expressed in the serum of NAFLD patients, and patients with HOMA-IR ≥2.5 had higher level of miR-129-5p than those with HOMA-IR <2.5. miR-129-5p had the ability to differentiate NAFLD patients from healthy individuals and might be associated with the development of IR. Serum miR-129-5p was positively correlated with the levels of HOMA-IR, BMI, total cholesterol (TC), and triglyceride (TG) in NAFLD patients. Downregulation of miR-129-5p regulates lipid metabolism and insulin sensitivity in NAFLD mice model. MiR-129-5p was upregulated in NAFLD patients and might be a potential diagnostic biomarker. The regulatory effect of miR-129-5p on NAFLD may function by regulating lipid accumulation and insulin sensitivity.

中文翻译：

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miR-129-5p 在非酒精性脂肪肝中的临床价值和作用

本研究旨在评估miR-129-5p在非酒精性脂肪肝（NAFLD）患者中的表达及其临床价值，并探讨其对胰岛素抵抗（IR）的调节作用。共纳入 117 名 NAFLD 患者和 110 名健康对照。通过qRT-PCR检测miR-129-5p的水平。为了评估 miR-129-5p 对 NAFLD 的诊断价值，建立了受试者工作特征曲线（ROC）。C57Bl/6小鼠高脂饮食建立NAFLD模型。进行腹腔内胰岛素耐受试验（IPITT）以评估 miR-129-5p 对 NAFLD 动物模型中 IR 的影响。miR-129-5p在NAFLD患者血清中高表达，HOMA-IR≥2.5的患者miR-129-5p水平高于HOMA-IR<2.5的患者。miR-129-5p 具有区分 NAFLD 患者和健康个体的能力，并且可能与 IR 的发展有关。NAFLD患者血清miR-129-5p与HOMA-IR、BMI、总胆固醇（TC）和甘油三酯（TG）水平呈正相关。miR-129-5p 的下调调节 NAFLD 小鼠模型中的脂质代谢和胰岛素敏感性。MiR-129-5p 在 NAFLD 患者中上调，可能是一种潜在的诊断生物标志物。miR-129-5p 对 NAFLD 的调节作用可能通过调节脂质积累和胰岛素敏感性发挥作用。miR-129-5p 的下调调节 NAFLD 小鼠模型中的脂质代谢和胰岛素敏感性。MiR-129-5p 在 NAFLD 患者中上调，可能是一种潜在的诊断生物标志物。miR-129-5p 对 NAFLD 的调节作用可能通过调节脂质积累和胰岛素敏感性发挥作用。miR-129-5p 的下调调节 NAFLD 小鼠模型中的脂质代谢和胰岛素敏感性。MiR-129-5p 在 NAFLD 患者中上调，可能是一种潜在的诊断生物标志物。miR-129-5p 对 NAFLD 的调节作用可能通过调节脂质积累和胰岛素敏感性发挥作用。