Microchimica Acta ( IF 5.3 ) Pub Date : 2021-09-16 , DOI: 10.1007/s00604-021-04988-7 Xinrong Shao 1, 2 , Xianzhen Song 1, 2 , Xin Liu 1, 2 , Liangguo Yan 3 , Lei Liu 1, 2 , Dawei Fan 1, 2 , Qin Wei 1, 2 , Huangxian Ju 1, 2, 4
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A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). CeO2-Au@Pt composed of sea urchin-like Au@Pt nanoparticles coated on CeO2 hollow nanospheres was immobilized on electrode surface to electrochemically catalyze H2O2 to produce a large number of superoxide anion (O2•−). The immunosensor was prepared by linking the capture antibody on immobilized CeO2-Au@Pt with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au star@BSA was used to bind abundant luminol for labeling the secondary antibody (Ab2). Upon the sandwich-typed immunoreactions, the O2•− could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low H2O2 concentration and demonstrated its high application potential in the clinical assay.
Graphical abstract
中文翻译：
基于核壳CeO2-Au@Pt纳米球的双信号放大电化学发光免疫传感器检测降钙素原
制造了一种双信号放大夹心电化学发光 (ECL) 免疫传感器，用于痕量检测降钙素原 (PCT)。将CeO 2 -Au@Pt 由包覆在CeO 2中空纳米球上的海胆状Au@Pt纳米粒子组成，将其固定在电极表面，电化学催化H 2 O 2产生大量超氧阴离子(O 2 •- )。通过将固定化CeO 2 -Au@Pt上的捕获抗体与七肽（HWRGWVC）连接制备免疫传感器，该七肽可以保持抗体的活性。制备的 Au star@BSA 用于结合丰富的鲁米诺标记二抗（Ab 2）。在夹心型免疫反应中，O 2 •-可以与免疫传感器表面引入的鲁米诺反应，产生强ECL强度。凭借出色的线性检测范围和 17 fg/mL 的低检测限，ECL 免疫传感器允许在低 H 2 O 2浓度下对 PCT 进行超灵敏检测，并证明了其在临床检测中的高应用潜力。
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