Hereditary spherocytosis (HS) is the most common inherited hemolytic anemia. The flow cytometric test using eosin-5′maleimide (EMA) is a well-established diagnostic method. However, in order to improve HS detection, it is recommended that EMA and an osmotic fragility test (OFT) both be performed. OFT is time consuming and labor intensive. We used a flow cytometric (FOFT) adaptation of the classical OFT reported by Yamamoto. We compare the FOFT to the classical OFT including practical data and propose options for simplifying this method.

中文翻译：

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改良流式细胞仪渗透脆性试验与经典方法诊断遗传性球形红细胞增多症的比较

遗传性球形红细胞增多症（HS）是最常见的遗传性溶血性贫血。使用 eosin-5'马来酰亚胺 (EMA) 的流式细胞仪检测是一种行之有效的诊断方法。然而，为了改进 HS 检测，建议同时进行 EMA 和渗透脆性试验 (OFT)。OFT 费时费力。我们使用了 Yamamoto 报告的经典 OFT 的流式细胞仪 (FOFT) 改编。我们将 FOFT 与包括实际数据的经典 OFT 进行比较，并提出简化该方法的选项。