Epoxide hydrolases are ubiquitous in nature and are utilized to catalyze the cofactor-independent hydrolysis of epoxides to their corresponding diols. These enzymes have tremendous potential and have been applied in the synthesis of bulk and fine chemical industry and utilized as chiral building blocks. Herein, we report a green, facile, and economical method for immobilization of epoxide hydrolase based on biomimetic mineralization. The organic-inorganic hybrid nanoflowers have received tremendous attention due to their higher catalytic activity and stability. The nanoflowers were synthesized, with the organic component being enzyme epoxide hydrolase and the inorganic component being Ca²⁺ ions. A unique hierarchical flower-like spherical structure with hundreds of spiked petals was observed. The synthesized nanoflowers were applied for styrene oxide hydrolysis, producing 1-phenyl-1,2-ethanediol. Further, the factors influencing the morphology, catalytic activity, and stability studies were performed to study the activity recovery of the synthesized organic-inorganic hybrid epoxide hydrolase nanoflowers. The findings will have interesting applications.

环氧化物水解酶在自然界中无处不在，用于催化不依赖辅因子的环氧化物水解为其相应的二醇。这些酶具有巨大的潜力，已应用于大宗和精细化工的合成，并用作手性构建块。在此，我们报告了一种基于仿生矿化固定环氧化物水解酶的绿色、简便和经济的方法。有机-无机杂化纳米花因其较高的催化活性和稳定性而受到极大关注。合成纳米花，有机成分为环氧化物水解酶，无机成分为Ca ²⁺离子。观察到具有数百个尖刺花瓣的独特分层花状球形结构。合成的纳米花用于氧化苯乙烯水解，产生 1-苯基-1,2-乙二醇。此外，对影响形态、催化活性和稳定性的因素进行了研究，以研究合成的有机-无机杂化环氧化物水解酶纳米花的活性恢复。这些发现将有有趣的应用。