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The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase
International Journal of Biological Macromolecules ( IF 8.2 ) Pub Date : 2021-09-16 , DOI: 10.1016/j.ijbiomac.2021.09.073
Anastasiya A Kasimova 1 , Nikolay P Arbatsky 1 , Olga Y Timoshina 2 , Mikhail M Shneider 2 , Alexander S Shashkov 1 , Alexander O Chizhov 1 , Anastasiya V Popova 3 , Ruth M Hall 4 , Johanna J Kenyon 5 , Yuriy A Knirel 1
Affiliation  

The KL26 gene cluster responsible for the synthesis of the K26 capsular polysaccharide (CPS) of Acinetobacter baumannii includes rmlBDAC genes for l-rhamnose (l-Rhap) synthesis, tle to generate 6-deoxy-l-talose (l-6dTalp) from l-Rhap, and a manC gene for D-mannose (D-Manp) that is rare in Acinetobacter CPS. K26 CPS material was isolated from A. baumannii isolate KZ-1098, and studied by sugar analysis, Smith degradation, and one and two-dimensional 1H and 13C NMR spectroscopy before and after O-deacetylation with aqueous ammonia. The following structure of the branched hexasaccharide repeating unit of the CPS was established:

2)βDManp14βDGlcp13αL6dTalp13βDGlcpNAc(1314AcαLRhap21αDGlcp

The structural depolymerase of phage vB_AbaP_APK26 cleaved selectively the β-GlcpNAc-(1 → 2)-α-Manp linkage in the K26 CPS formed by WzyK26 to give monomer, dimer, and trimer of the CPS repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as 1H and 13C NMR spectroscopy. The wzyK26 gene responsible for this linkage and the manC gene were only found in six A. baumannii genomes carrying KL26 and one carrying the novel KL148 gene cluster, indicating the rare occurrence of β-GlcpNAc-(1 → 2)-α-Manp in A. baumannii CPS structures. However, K26 shares a β-d-Glcp-(1 → 3)-α-l-6dTalp-(1 → 3)-β-d-GlcpNAc trisaccharide fragment with a group of related A. baumannii CPSs that have varying patterns of acetylation of l-6dTalp.



中文翻译:

鲍曼不动杆菌 KZ-1098 的 K26 荚膜多糖:特定噬菌体解聚酶的结构和裂解

负责的K26荚膜多糖(CPS)的合成KL26基因簇鲍曼不动杆菌包括rmlBDAC基因鼠李糖(-RHA p)的合成,TLE,以产生6-脱氧-talose(-6dTal p)从-RHA pmanC基因为d -甘露糖(d -Man p),其是在罕见的不动杆菌属CPS。K26 CPS 材料是从鲍曼不动杆菌中分离出来的分离KZ-1098,并通过糖分析、Smith降解以及用氨水O-脱乙酰前后的一维和二维1 H和13 C NMR光谱研究。建立了以下 CPS 支链六糖重复单元的结构:

2)-β-D-男子p-14-β-D-葡萄糖p-13-α--6塔尔p-13-β-D-葡萄糖p醋酸钠-(1314交流电α--p-21-α-D-葡萄糖p

噬菌体 vB_AbaP_APK26 的结构解聚酶选择性裂解Wzy K26形成的 K26 CPS 中的 β-Glc p NAc-(1 → 2)-α-Man p键,得到 CPS 重复单元的单体、二聚体和三聚体,分别为通过高分辨率电喷雾电离质谱以及1 H 和13 C NMR 光谱进行表征。负责这种连锁的wzy K26基因和manC基因仅在六个携带 KL26 的鲍曼不动杆菌基因组和一个携带新的 KL148 基因簇中发现,表明 β-Glc p NAc-(1 → 2)-α的罕见发生-Man p in鲍曼不动杆菌CPS 结构。然而,K26与一组相关的鲍曼不动杆菌CPS共享一个 β- d -Glc p -(1 → 3)-α- l -6dTal p -(1 → 3)-β- d -Glc p NAc 三糖片段l -6dTal p具有不同的乙酰化模式。

更新日期:2021-09-21
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