Traditional pretreatment of lignocellulosic biomass is often accompanied by washing and disposal of wastewater, which leads to overuse of water and loss of by-products. The objectives of this study were to validate the potential of an acid-base integrated process for simultaneous sugars, furans, and lignin production without washing and wastewater discarding. The difference in conversion performance among different biomass resources was also demonstrated. Parallel acetic acid (HOAc, pH = 2.25) and sodium hydroxide (NaOH, pH = 13.46) pretreatments followed by solid and liquid integration were applied to four genotypes of industrial hemp (Cannabis sativa L.) biomass that were harvested from two planting locations (Haysville and Manhattan, KS). Results showed that genotype, planting location, and their interaction had notable influences on biomass composition and its conversion to bioproducts but exhibited different trends. Glucan content of biomass from Haysville, ranging from 47.29 to 50.05%, were higher than those of 42.49–48.38 % from Manhattan with the lowest being Vega (Manhattan) and the highest being Hlukouskii (Haysville). Xylan and lignin contents in all the hemp genotypes were 11.70–13.88 % and 10.45–15.14 %, respectively. The integration process effectively rendered the pH of the integrated filtrate and slurry to approximately 4.80. The highest lignin recovery of 73.13 g/kg biomass was achieved by Rigel from Manhattan. Fourier transform infrared spectroscopy (FTIR) characterization showed that only lignin derived from Vega (Haysville) and Anka (Manhattan) was comparable to the commercial alkali lignin. Retaining monosaccharides (2.24–3.81 g/L) enhanced sugar concentrations (glucose: 40.40–45.71 g/L; xylose: 7.09–8.88 g/L) and conversion efficiencies (glucose: 71.19–77.71 %; xylose: 45.42–52.03 %). Besides, furans including 0.79–1.25 g/L of hydroxymethylfurfural (HMF) and 0.99–1.59 g/L of furfural coupling with 1.96–2.95 % and 10.00–14.65 % conversion efficiencies, respectively, were obtained in the final hydrolysate. Biomass from Haysville produced relatively higher glucose concentrations than those from Manhattan. Based on mass balance, the most productive genotype was Rigel. This study offers essential information to reduce water and chemical overconsumption and to understand the effects of genotype and planting location on biomass valorization.

木质纤维素生物质的传统预处理通常伴随着废水的洗涤和处理，这导致水的过度使用和副产品的损失。本研究的目的是验证酸碱一体化工艺在无需洗涤和废水排放的情况下同时生产糖、呋喃和木质素的潜力。还证明了不同生物质资源之间转化性能的差异。平行乙酸 (HOAc, pH = 2.25) 和氢氧化钠 (NaOH, pH = 13.46) 预处理，然后固液整合应用于四种基因型工业大麻 ( Cannabis sativaL.) 从两个种植地点（海斯维尔和曼哈顿，堪萨斯州）收获的生物量。结果表明，基因型、种植地点及其相互作用对生物量组成及其向生物产品的转化有显着影响，但表现出不同的趋势。海斯维尔生物量的葡聚糖含量为 47.29% 至 50.05%，高于曼哈顿的 42.49-48.38%，最低的是织女星（曼哈顿），最高的是赫卢库斯基（海斯维尔）。所有大麻基因型的木聚糖和木质素含量分别为 11.70-13.88% 和 10.45-15.14%。整合过程有效地使整合的滤液和浆液的 pH 值达到约 4.80。来自曼哈顿的 Rigel 实现了 73.13 g/kg 生物质的最高木质素回收率。傅里叶变换红外光谱 (FTIR) 表征表明，只有源自 Vega (Haysville) 和 Anka (Manhattan) 的木质素与商业碱性木质素相当。保留单糖 (2.24–3.81 g/L) 可提高糖浓度（葡萄糖：40.40–45.71 g/L；木糖：7.09–8.88 g/L）和转化效率（葡萄糖：71.19–77.71%；木糖：525.32%） . 此外，在最终水解产物中获得了含有 0.79-1.25 g/L 羟甲基糠醛 (HMF) 和 0.99-1.59 g/L 糠醛偶联物的呋喃，转化率分别为 1.96-2.95% 和 10.00-14.65%。海斯维尔的生物质产生的葡萄糖浓度比曼哈顿的高。基于质量平衡，最具生产力的基因型是 Rigel。