Smallmouth bass in the Susquehanna River Basin, Chesapeake Bay Watershed, USA, have been exhibiting clinical signs of disease and reproductive endocrine disruption (e.g., intersex, male plasma vitellogenin) for over fifteen years. Previous histological and targeted chemical analyses have identified infectious agents and pollutants in fish tissues including organic contaminants, mercury, and perfluorinated compounds, but a common causative link for the observed signs of disease across this widespread area has not been determined. This study examines 146 young-of-year smallmouth bass collected from 14 sampling sites in the Susquehanna River Basin, Pennsylvania, USA with varying levels of disease prevalence. Whole fish were extracted by a recently developed modification to the quick, easy, cheap, effective, rugged, and safe extraction method and analyzed by comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry. A targeted analysis was conducted to identify the presence and quantity of 127 known contaminants, including polychlorinated biphenyls, brominated diphenyl ethers, organochlorinated pesticides, and pharmaceutical and personal care products. A non-targeted analysis was conducted on the same data set to identify analytes of interest not included on routine target compound lists. Chromatographic alignment through Statistical Compare (ChromaTOF GC) was followed by Fisher ratio and principal component analysis to reduce the data set from thousands of peaks per sample to a final data set of 65 analytes of interest. Comparisons of these 65 compounds between Normal (no observed health anomalies) and Lesioned (observed health anomaly at time of collection) fish revealed increased levels of three chemical families in Lesioned fish including esters, ketones, and nitrogen containing compounds.

美国切萨皮克湾流域萨斯奎哈纳河流域的小嘴鲈鱼已经表现出疾病和生殖内分泌紊乱（例如双性人、雄性血浆卵黄蛋白原）的临床症状超过 15 年。先前的组织学和靶向化学分析已经确定了鱼类组织中的传染性病原体和污染物，包括有机污染物、汞和全氟化合物，但尚未确定在这一广泛区域观察到的疾病迹象的常见因果关系。本研究检查了从美国宾夕法尼亚州萨斯奎哈纳河流域的 14 个采样点收集的 146 条幼年小嘴鲈鱼，这些地点的疾病流行程度各不相同。整条鱼是通过最近开发的改进方法提取的，该方法快速、简单、便宜、有效、坚固、和安全的提取方法，并通过综合二维气相色谱-飞行时间质谱联用进行分析。进行了有针对性的分析，以确定 127 种已知污染物的存在和数量，包括多氯联苯、溴化二苯醚、有机氯化农药以及医药和个人护理产品。对同一数据集进行了非靶向分析，以识别未包含在常规目标化合物列表中的目标分析物。通过统计比较 (ChromaTOF GC) 进行色谱比对后，进行 Fisher 比率和主成分分析，以将数据集从每个样品的数千个峰减少到包含 65 种目标分析物的最终数据集。