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Simultaneous detection of phenacetin and paracetamol using ELISA and a gold nanoparticle-based immunochromatographic test strip
Analyst ( IF 3.6 ) Pub Date : 2021-08-20 , DOI: 10.1039/d1an01173a Jingjing Yao 1, 2 , Xinxin Xu 1, 2 , Liqiang Liu 1, 2 , Hua Kuang 1, 2 , Zhengyou Wang 3 , Chuanlai Xu 1, 2
Analyst ( IF 3.6 ) Pub Date : 2021-08-20 , DOI: 10.1039/d1an01173a Jingjing Yao 1, 2 , Xinxin Xu 1, 2 , Liqiang Liu 1, 2 , Hua Kuang 1, 2 , Zhengyou Wang 3 , Chuanlai Xu 1, 2
Affiliation
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We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) and paracetamol (PAP) using an anti-PNCT monoclonal antibody (mAb). The sensitive anti-PNCT mAb (2D6) had a half maximal inhibitory concentration (IC50) and limit of detection (LOD) of 3.51 and 0.21 ng mL−1, respectively. Additionally, its cross-reactivity with PAP was approximately 10.1%. The developed GNP-ICS assay based on GNP-labeled mAb was sensitive for the detection of PNCT with vLOD and cut-off values of 2.5 and 50 ng mL−1 respectively and a vLOD value of 25 ng mL−1 for PAP. Furthermore, the developed icELISA and GNP-ICS assays were applied to determine PNCT-spiked beverage samples without pretreatment, in addition to a kind of PAP-containing drug. The recoveries were validated using high performance liquid chromatography (HPLC). The results revealed that the developed GNP-ICS assay was reliable for the detection of PNCT in practical samples.
中文翻译：
使用 ELISA 和基于金纳米颗粒的免疫色谱测试条同时检测非那西丁和扑热息痛
我们开发了一种灵敏且快速的基于金纳米颗粒的免疫层析条 (GNP-ICS)，用于使用抗 PNCT 单克隆抗体 (mAb) 检测非那西丁 (PNCT) 和扑热息痛 (PAP)。敏感的抗PNCT mAb (2D6) 的半数抑制浓度(IC 50 ) 和检测限(LOD) 分别为3.51 和0.21 ng mL -1。此外，它与 PAP 的交叉反应性约为 10.1%。基于 GNP 标记的 mAb 开发的 GNP-ICS 测定对于检测 PNCT 敏感，vLOD 和截断值分别为 2.5 和 50 ng mL -1以及PAP的 vLOD 值为 25 ng mL -1。此外，开发的ic除了一种含 PAP 的药物外，还应用 ELISA 和 GNP-ICS 测定来确定未经预处理的 PNCT 加标饮料样品。使用高效液相色谱 (HPLC) 验证回收率。结果表明，开发的 GNP-ICS 测定法对于检测实际样品中的 PNCT 是可靠的。
更新日期：2021-09-16
中文翻译：
使用 ELISA 和基于金纳米颗粒的免疫色谱测试条同时检测非那西丁和扑热息痛
我们开发了一种灵敏且快速的基于金纳米颗粒的免疫层析条 (GNP-ICS)，用于使用抗 PNCT 单克隆抗体 (mAb) 检测非那西丁 (PNCT) 和扑热息痛 (PAP)。敏感的抗PNCT mAb (2D6) 的半数抑制浓度(IC 50 ) 和检测限(LOD) 分别为3.51 和0.21 ng mL -1。此外，它与 PAP 的交叉反应性约为 10.1%。基于 GNP 标记的 mAb 开发的 GNP-ICS 测定对于检测 PNCT 敏感，vLOD 和截断值分别为 2.5 和 50 ng mL -1以及PAP的 vLOD 值为 25 ng mL -1。此外，开发的ic除了一种含 PAP 的药物外，还应用 ELISA 和 GNP-ICS 测定来确定未经预处理的 PNCT 加标饮料样品。使用高效液相色谱 (HPLC) 验证回收率。结果表明，开发的 GNP-ICS 测定法对于检测实际样品中的 PNCT 是可靠的。
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