Microsporum canis, a common pathogenic skin fungus, can cause dermatophytosis in humans and animals. Zinc is an important trace element and plays an important role in the growth and metabolism of fungi. Currently, the effects of zinc deficiency on growth, gene expression, and metabolic pathway have not been clarified in M. canis. Therefore, M. canis was cultured under zinc restriction, and RNA-Seq was conducted in this study. The growth of M. canis was severely inhibited, and many genes showed significant upregulation and downregulation in M. canis with zinc deficiency. Zinc deficiency could negatively affect the gene expression and biological metabolic pathway in M. canis. The zinc-responsiveness transcriptional activator (ZafA) gene was significantly upregulated and shared homology with Zap1. Thus, the ZafA gene might be the main transcription factor regulating M. canis zinc homeostasis. The ZafA gene knockout strain, ZafA-hph, was constructed via Agrobacterium tumefaciens-mediated transformation (ATMT) in M. canis for the first time to assess its function. In vitro growth ability, hair biodegradation ability, virulence test, and zinc absorption capacity in ZafA-hph and wild-type M. canis strains were compared. Results showed that the ZafA gene plays an important role in zinc absorption, expression of zinc transporter genes, and growth and pathogenicity in M. canis and can be used as a new drug target. Cutting off the zinc absorption pathway can be used as a way to prevent and control infection in M. canis.

犬小孢子菌是一种常见的致病性皮肤真菌，可引起人和动物的皮肤癣菌病。锌是一种重要的微量元素，在真菌的生长和代谢中起重要作用。目前，缺锌对生长、基因表达和代谢途径的影响尚未阐明。犬类. 所以，犬类在锌限制条件下培养，并在本研究中进行了 RNA-Seq。的成长犬类 被严重抑制，许多基因显示出显着的上调和下调 犬类缺锌。锌缺乏会对基因表达和生物代谢途径产生负面影响犬类. 锌反应性转录激活因子（扎法) 基因显着上调并与 Zap1. 就这样扎法 基因可能是主要的转录因子调节 犬类锌稳态。这扎法 构建基因敲除菌株ZafA-hph 通过根癌农杆菌介导转化（ATMT） 犬类 第一次评估其功能。 体外 ZafA-hph 和野生型的生长能力、毛发生物降解能力、毒力试验和锌吸收能力 犬类菌株进行了比较。结果表明，扎法 基因在锌的吸收、锌转运蛋白基因的表达以及生长和致病性中起重要作用。 犬类并可作为新的药物靶点。切断锌吸收途径可作为预防和控制感染的一种方法犬类.