The human oral microbiota plays a vital role in maintaining metabolic homeostasis. To explore the relationship between Helicobacter pylori (Hp) and reflux esophagitis, we collected 86 saliva samples from reflux esophagitis patients (RE group) and 106 saliva samples from healthy people (C group) for a high-throughput sequencing comparison. No difference in alpha diversity was detected between the RE and the C groups, but beta diversity of the RE group was higher than the C group. Bacteroidetes was more abundant in the RE group, whereas Firmicutes was more abundant in the C group. The linear discriminant analysis effect size analysis demonstrated that the biomarkers of the RE group were Prevotella, Veillonella, Leptotrichia, and Actinomyces, and the biomarkers of the C group were Lautropia, Gemella, Rothia, and Streptococcus. The oral microbial network structure of the C group was more complex than that of the RE group. Second, to explore the effect of Hp on the oral microbiota of RE patients, we performed the ¹⁴C-urea breath test on 45 of the 86 RE patients. We compared the oral microbiota of 33 Hp-infected reflux esophagitis patients (REHpp group) and 12 non-Hp-infected reflux esophagitis patients (REHpn group). No difference in alpha diversity was observed between the REHpn and REHpp groups, and beta diversity of the REHpp group was significantly lower than that of the REHpn group. The biomarkers in the REHpp group were Veillonella, Haemophilus, Selenomonas, Megasphaera, Oribacterium, Butyrivibrio, and Campylobacter; and the biomarker in the REHpn group was Stomatobaculum. Megasphaera was positively correlated with Veillonella in the microbial network of the REHpp group. The main finding of this study is that RE disturbs the human oral microbiota, such as increased beta diversity. Hp infection may inhibit this disorderly trend.

人类口腔微生物群在维持代谢稳态方面起着至关重要的作用。探究两者之间的关系幽门螺杆菌 (生命值）和反流性食管炎，我们收集了 86 份反流性食管炎患者（RE 组）的唾液样本和 106 份健康人（C 组）的唾液样本进行高通量测序比较。RE组和C组之间没有检测到α多样性差异，但RE组的β多样性高于C组。RE 组中拟杆菌更丰富，而 C 组中厚壁菌门更丰富。线性判别分析效应大小分析表明，RE 组的生物标志物是普雷沃泰拉, 韦洛内拉, 钩端螺旋体， 和 放线菌，C组的生物标志物是 劳托皮亚, 杰梅拉, 罗西亚， 和 链球菌. C组口腔微生物网络结构比RE组复杂。二、探索效果生命值针对 RE 患者的口腔微生物群，我们对 86 名 RE 患者中的 45 名进行了¹⁴ C-尿素呼气测试。我们比较了 33 个口腔微生物群生命值-感染的反流性食管炎患者（REHpp组）和12例非Hp感染的反流性食管炎患者（REHpn组）。REHpn组和REHpp组之间没有观察到α多样性差异，REHpp组的β多样性显着低于REHpn组。REHpp 组的生物标志物是韦洛内拉, 嗜血杆菌, 硒单胞菌, 大球藻, 产肠杆菌, 丁酸弧菌， 和 弯曲杆菌; REHpn 组的生物标志物是口腔杆菌. 大球藻 正相关 韦洛内拉在 REHpp 组的微生物网络中。这项研究的主要发现是，RE 扰乱了人类口腔微生物群，例如增加了 β 多样性。生命值 感染可能会抑制这种无序的趋势。